异对大鼠脊髓神经元NMDA受体介导反应的突触效应
Kiku Nonaka1, Michiko Nakamura2, Mami Noda3
1Research Division for Life Science, Kumamoto Health Science University, 325 Izumi-machi, Kita-ku, Kumamoto 861-5598, Japan.
Neuroscience letters
|June 24, 2024
概括
(Xe) 抑制脊髓神经元中的NMDA受体活性. 这项研究发现,Xe减少了自发和唤起的NMDA受体电流,这表明了前突触和后突触抑制机制.
科学领域:
- 神经科学是一个神经科学.
- 药理学 药理学是指药理学的学科.
背景情况:
- 激发性突触传输对神经元功能至关重要.
- NMDA受体在调解刺激性神经传递中发挥着关键作用.
- 克森对神经元信号的调节作用尚未完全理解.
研究的目的:
- 为了阐明 (Xe) 调节NMDA受体介导的突触传输的精确机制.
- 研究Xe对脊髓神经元中NMDA受体介导的自发和唤起的刺激后突触电流 (EPSCs) 的影响.
主要方法:
- 在单个单独的脊柱神经背部委托核 (SDCN) 神经元上进行了全细胞补丁记录.
- 分析了由AMPA/KA和NMDA受体介导的自发和唤起的刺激后突触电流 (s/eEPSCs).
- 在 Xe 应用之前和之后比较了 s/eEPSC 的动力性质.
主要成果:
- 与AMPA/KA受体介导的EPSC相比,NMDA受体介导的EPSC (s/eEPSC_NMDA) 的动力表现较慢.
- Xe显著降低了自发NMDA受体EPSCs的频率和幅度.
- Xe降低了振幅,并增加了唤起的NMDA受体EPSC的失败率和配对脉冲比,而不会改变衰变时间常数.
结论:
- 克森抑制了NMDA受体介导的突触传输.
- Xe通过前突触 (减少释放概率) 和后突触 (减少NMDA受体功能) 机制发挥作用.
- 这些发现提供了关于的神经保护潜力的见解.
更多相关视频
08:30Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons
Published on: January 18, 2019
14.1K
08:38Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals
Published on: May 25, 2011
15.6K
相关概念视频
01:30Ligand-Gated Ion Channel Receptor: Gating Mechanism
2.2K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
2.2K
01:26Chemical Synapses
8.8K
Chemical synapses are specialized sites between two neurons or between a neuron and a non-neuronal cell like a muscle, glandular or sensory cell.
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
8.8K
01:28Integration of Synaptic Events
1.5K
Synaptic integration mainly includes the summation of graded potentials. Graded potentials, regardless of their type, cause subtle alterations in membrane voltage, resulting in either depolarization or hyperpolarization. These incremental changes, when combined or summed, can propel the neuron toward its threshold. Consider, for example, a membrane experiencing a +15 mV shift, causing it to depolarize from -70 mV to -55 mV. In this scenario, graded potentials govern the membrane's ability to...
1.5K