[对1,4-环二甲基胺的酶性生产]
Yeting Han1, Zhizhen He1, Wanqing Wei2,3
1School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, Jiangsu, China.
概括
使用工程酶的新型生物基途径将1,4-环二甲基化物转化为1,4-环二甲基胺 (1,4-BAC). 这种更绿色的方法为生物基材料的传统化学合成提供了一个有希望的替代方案.
科学领域:
- 生物催化剂和代谢工程
- 绿色化学和可持续合成
- 材料科学和聚合物化学
背景情况:
- 1,4-环二甲基胺 (1,4-BAC) 是生物基材料的关键单体,具有多种应用.
- 目前1,4-BAC的化学合成方法涉及昂贵的催化剂,恶劣的条件和安全问题.
- 对于1,4-BAC生产来说,有必要找到可持续和更安全的替代品.
研究的目的:
- 开发一种更绿色的,基于酶的级联转化途径,用于合成1,4-BAC.
- 改造关键酶以提高其活性和效率.
- 优化反应条件以最大限度地提高产品产量和转化率.
主要方法:
- 通过使用来自大肠杆菌* (氨基转移酶,Ec*TA),大肠杆菌* (谷氨酸脱酶,ScGlu-DH) 和Candida boidinii* (形式脱酶,Cb*FDH) 的酶构建了一个三种酶级联路径.
- 使用结构指导蛋白质工程来提高*Ec*TA的性能,产生有益的突变物*Ec*TAF91Y.
- 构建了重组菌株,并优化了反应条件,以最大限度地将1,4-环二氧化碳化物转化为1,4-BAC.
主要成果:
- 与野生类型相比,工程 *Ec*TAF91Y 突变体的特定活动增加了2.2倍, *k*cat/Km 增加了1.9倍.
- 在优化条件下,基质度为40g/L,产生了1,4-BAC的 (27.4±0.9) g/L.
- 该过程实现了目标产品的67.5%±2.1%的摩尔转换率.
结论:
- 一种新且高效的两种细菌,三种酶级联通路已成功建立,用于生物基合成1,4-BAC.
- 蛋白质工程显著提高了该途径中关键酶的催化效率.
- 这种生物催化方法为生产有价值的生物基单体提供了传统化学合成方法的可持续和潜在可扩展的替代方案.
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