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循环RNA circLIFR通过调节miR-429/TIMP2轴来抑制乳头甲状腺癌的进展

Fengyuan Zhang¹, Jiazheng Li¹, Jingjing Xu¹

¹Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, 212013, China.

Journal of cancer research and clinical oncology

|June 24, 2024

概括

循环RNA LIF受体亚单元α (circLIFR) 在乳头甲状腺癌 (PTC) 中起到瘤抑制作用. 它通过调节miR-429/TIMP2通路来抑制PTC进展,提供了一个潜在的治疗标.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

循环RNAs (circRNAs) 在各种癌症中至关重要,包括乳头甲状腺癌 (PTC).
在PTC进展中,circLIF受体亚单元α (circLIFR) 的特定作用尚不清楚.

研究的目的:

为了研究circLIFR在乳头甲状腺癌中的功能和机制.
为了确定PTC中circLIFR,miR-429和TIMP2之间的关系.

主要方法:

使用定量PCR和西式斑点测试来测量circLIFR,miR-429和TIMP2水平.
进行了细胞增殖,迁移和入侵试验,以评估circLIFR和miR-429.9的功能作用.
双露西法酶记者,RNA免疫沉和FISH测定证实了circLIFR,miR-429和TIMP2之间的相互作用.

主要成果:

在PTC组织和细胞中,circLIFR和TIMP2水平下降,而miR-429水平增加.
循环LIFR过度表达抑制了PTC细胞的增殖和迁移,而循环LIFR的淘汰促进了这些过程.
circLIFR作为miR-429的海绵,从而调节TIMP2的表达,并在体内抑制瘤生长.

结论:

circLIFR通过抑制通过miR-429/TIMP2轴的进展,在PTC中起到瘤抑制作用.
circLIFR显示出作为乳头甲状腺癌治疗的新型治疗点的潜力.

关键词:

在CircLIFR中,循环的RNA是循环的RNA.乳头甲状腺癌是一种乳头甲状腺癌.时间表2 TIMP2 在 miR-42929 中使用.

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