采用料批量策略,完全消除 ABE 发酵过程中由林氏纤维素衍生的抑制剂的负面影响
Barbora Branska1, Kamila Koppova2, Marketa Husakova2
1Department of Biotechnology, University of Chemistry and Technology Prague, Technická 5, 16628, Prague, Czech Republic. barbora.branska@vscht.cz.
Biotechnology for biofuels and bioproducts
|June 24, 2024
概括
一种新的养策略有效地减轻了在乙-布坦醇-乙醇 (ABE) 发酵中使用的纤维素酶抑制剂. 这种方法可以实现强大的生物工艺控制,克服用于化学生产的纤维素蛋白利用的主要瓶.
科学领域:
- 生物技术是生物技术.
- 生物化学工程 生物化学工程
- 微生物发酵 微生物发酵
背景情况:
- 纤维素生物质处理释放抑制剂,阻碍生物技术化学品的生产.
- 原始的,未经排毒的红纤维素水解剂和特定的抑制剂在乙-布坦醇-乙醇 (ABE) 发酵中进行了测试.
- 使用Clostridium beijerinckii NRRL B-598作为生产菌株.
研究的目的:
- 证明正确的养策略可以减轻由林氏纤维素衍生的抑制剂的负面影响.
- 为了优化料批发发酵以生产ABE,使用有毒的纤维素基解液.
- 为了研究通过Clostridium beijerinckii对酸的排毒.
主要方法:
- 用三种养方案进行料批发发酵,使用原始,未经排毒的纤维素酸盐解剂.
- 种植Clostridium beijerinckii NRRL B-598.8. 这种植物的种植方式
- 在TYA介质中的ABE发酵,补充了ferulic和cumaric酸.
主要成果:
- 对抑制剂的C. beijerinckii敏感性随生长阶段而变化,在早期酸性发生过程中最为关键.
- 在代谢开关附近开始的养产生了稳定,高的ABE产量,完全使用了葡萄糖和88%的树脂糖.
- 酸和酸被克洛斯特里迪亚代谢排毒,最好的养策略增加了500%的布坦醇标位.
结论:
- 定时的连续养可以防止酸崩,并使有毒基质利用.
- 适当的生物技术过程控制策略可以完全消除林氏纤维素抑制剂的负面影响.
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