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对一个无序的蛋白质和一个折叠的RNA之间的相互作用的分子见解

Rishav Mitra^1,2, Emery T Usher^3,4, Selin Dedeoğlu⁵

¹Howard Hughes Medical Institute, University of Michigan, Ann Arbor, MI 48109, USA.

bioRxiv : the preprint server for biology

|June 25, 2024

概括

本质上有障碍的蛋白质SERF与HIV-1 TAR RNA结合,但没有获得结构,显示了适度的紧缩. 这种由电荷驱动的相互作用会影响蛋白质-RNA复合体的形成和生物分子凝聚剂组合.

科学领域:

生物化学生物化学
分子生物学分子生物学
生物物理学的生物物理.

背景情况:

内在无序区域 (IDR) 对于蛋白质相互作用和生物分子凝聚物形成至关重要.
RNA结合蛋白 (RBPs) 使用IDR与结构域一起进行它们的功能.
了解IDR-RNA动态是阐明复杂生物过程的关键.

研究的目的:

描述内在无序蛋白质SERF.的RNA结合特性.
调查SERF与HIV-1 TAR RNA结合时发生的结构和动态变化.
探索SERF-RNA相互作用在相分离和复杂形成中的作用.

主要方法:

使用了溶液状态生物物理技术 (例如,NMR,光谱学).
用分子模拟来补充实验数据.
单独对SERF进行了表征,并与TAR RNA复合进行了表征.

主要成果:

对TAR RNA的SERF结合并没有诱导任何分子的显著结构变化.
在RNA结合时观察到SERF组合的适度全球紧缩.
由于RNA相互作用,在SERF内部推断了减弱的电荷排斥.
在高度下,SERF-RNA系统表现出电荷驱动的关联相分离.

结论:

SERF-RNA相互作用为研究IDR-RNA动态提供了一个模型.
了解这些相互作用可以更深入地了解复杂的形成和液体-液体相分离.
这些发现为在RNA的存在下SERF的更高阶组装提供了理由.

关键词:

这是一种RNA结合蛋白.RNA-蛋白质复合体是一种 RNA-蛋白质复合体.凝结剂是一种凝结剂.本质上是无序的区域 (IDR).核磁共振 (NMR) 是一种核磁共振技术.阶段分离的阶段分离.

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