相关实验视频

Updated: Jun 23, 2025

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
11:38

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Published on: November 26, 2018

10.4K

通过PARP-DUX4调控轴向功能人类全能干细胞状态进行蛋白基因组重编程

Ludovic Zimmerlin, Ariana Angarita, Tea Soon Park

    bioRxiv : the preprint server for biology
    |June 25, 2024
    PubMed
    概括

    人类多能干细胞通过抑制PARP1和Tankyrases被重新编程成全能干细胞. 这些细胞有助于所有胚胎和胚胎外的基因系,为早期人类发育提供了一个模型.

    更多相关视频

    Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets07:08

    Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets

    Published on: February 2, 2024

    775
    A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells08:01

    A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells

    Published on: August 29, 2020

    2.3K

    相关概念视频

    Somatic to iPS Cell Reprogramming01:29

    Somatic to iPS Cell Reprogramming

    Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
    2.2K
    Methods of Nuclear Reprogramming01:24

    Methods of Nuclear Reprogramming

    Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
    1.8K
    Chromatin Modification in iPS Cells01:32

    Chromatin Modification in iPS Cells

    Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
    Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
    1.6K
    Introduction to Nuclear Reprogramming01:14

    Introduction to Nuclear Reprogramming

    Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
    1.9K
    Combinatorial Gene Control02:33

    Combinatorial Gene Control

    Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
    The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
    8.3K
    Induced Pluripotent Stem Cells01:06

    Induced Pluripotent Stem Cells

    Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
    Somatic...
    4.0K