Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Alternative RNA Splicing02:18

Alternative RNA Splicing

21.1K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Combinatorial Targeting of Avapritinib-Driven MAP Kinase Activation in High-Grade Glioma.

Research square·2026
Same author

Retraction Note: NSD2 targeting reverses plasticity and drug resistance in prostate cancer.

Nature·2026
Same author

Dual-chirality flexagon linkages with infinite eversion and surface reconfigurability.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Targeting of MEK and Autophagy in Pancreatic Adenocarcinoma and Analysis of Treatment Sensitivity in Preclinical and Clinical Models: MEKiAUTO.

JCO precision oncology·2026
Same author

Intrathecal Administration of Riluzole in Amyotrophic Lateral Sclerosis.

Neurosurgery·2026
Same author

Onconeural antigen NOVA1 dysregulates RNA alternative splicing in breast cancer.

RNA (New York, N.Y.)·2026
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026
Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026
查看所有相关文章

相关实验视频

Updated: Jun 23, 2025

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing
06:38

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

Published on: October 12, 2018

18.8K

反向工程神经元类型特定和类型直角拼接调节网络使用单细胞转录组.

Daniel F Moakley1,2,3, Melissa Campbell1,2,3,4, Miquel Anglada-Girotto1,5

  • 1Department of Systems Biology, Columbia University, New York, NY 10032, USA.

bioRxiv : the preprint server for biology
|June 25, 2024
PubMed
概括
此摘要是机器生成的。

研究人员在133种小鼠神经元类型中绘制了替代拼接 (AS) 监管格局. 他们确定了关键的RNA结合蛋白 (RBPs),驱动神经元身份和特定的拼接程序,揭示了基因表达之外的分子多样性.

关键词:
第122章 没有了RNA拼接规则的调节RNA结合蛋白质是RNA结合的蛋白质.网络推断 网络推断神经元亚型的神经元亚型

更多相关视频

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq
07:49

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq

Published on: October 26, 2018

9.5K
Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
11:26

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Published on: May 22, 2017

13.8K

相关实验视频

Last Updated: Jun 23, 2025

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing
06:38

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

Published on: October 12, 2018

18.8K
Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq
07:49

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq

Published on: October 26, 2018

9.5K
Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
11:26

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Published on: May 22, 2017

13.8K

科学领域:

  • 神经科学是一个神经科学.
  • 基因组学就是基因组学.
  • 分子生物学分子生物学

背景情况:

  • 替代拼接 (AS) 产生各种基因异型,对不同类型的神经元至关重要.
  • 目前对RNA结合蛋白 (RBPs) 和AS的研究仅限于少数神经元类型,需要全面建模.

研究的目的:

  • 创建神经元类型特定的AS监管格局的整体地图.
  • 识别定义神经元身份和多样性的RBPs和拼接模块.

主要方法:

  • 网络逆向工程应用于来自133种小鼠新皮层细胞类型的单细胞转录组.
  • 350个RBP的推断调节和它们的细胞类型特定活动.
  • 在体外验证RBP功能使用ESC差异化系统.

主要成果:

  • 成功地绘制了AS监管格局,并推断出350个RBP规则与细胞类型特定活动.
  • 确定了Elavl2作为一个关键的RBP,调节GABAergic内部神经元中的MGE特定拼接.
  • 在各种神经元类中发现了特定于长短投射神经元的外子和调节器模块.

结论:

  • 这项研究为了解驱动神经元分子多样性的拼接监管程序提供了宝贵的资源.
  • 神经元身份是由AS监管程序塑造的,其中一些是独立于基因表达分类的.
  • 这项工作促进了对替代拼接如何为神经系统的复杂性做出贡献的理解.