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内皮细胞Piezo1促进血管光滑肌肉细胞在大动脉上的分化

Javier Abello¹, Ying Yin¹, Yonghui Zhao²

¹Department of Cell Biology and Physiology, Washington University in St. Louis School of Medicine, St. Louis, MO, USA.

bioRxiv : the preprint server for biology

|June 25, 2024

概括

机械敏感的离子通道Piezo1通过引导血管光滑肌细胞 (vSMCs) 来帮助稳定动脉. 皮埃佐1在内皮细胞中的活性感知到血液流动的力量,从而影响静脉中枢的分化和动脉壁的发育.

科学领域:

心血管生物学心血管生物学
机械生物学机械生物学
发展生物学发展生物学

背景情况:

血管稳定是一个由血液流动影响的关键机械敏感过程.
血管光滑肌细胞 (vSMCs) 在动脉发育和稳定中发挥着关键作用.

研究的目的:

调查机械敏感离子通道Piezo1在调节斑马鱼发育期间血管光滑肌细胞 (vSMC) 向的作用.
为了确定Piezo1在哺乳动物血管发育中的功能性保存.

主要方法:

在斑马鱼中利用小分子Piezo1激动剂和对抗剂来调节通道活性.
在斑马鱼体内进行了Piezo1和Piezo2的基因抑制.
生成内皮细胞特异的Piezo1淘汰小鼠.
进行了体外建模试验,以评估内皮细胞对流动力的反应.

主要成果:

调节Piezo1活动改变了斑马鱼的klf2a水平和vSMC与动脉和静脉的关联.
斑马鱼中Piezo1/2的遗传抑制导致背上大动脉上vSMCs的损失.
在小鼠体内皮细胞特异的Piezo1淘汰法复制斑马鱼的发现,减少vSMC积累.
实验室试验表明,内皮细胞对流动力的差异感知会影响壁细胞分化基因.

结论:

内皮细胞Piezo1对于感知动脉血流力至关重要.
在发育过程中,Piezo1调解壁细胞分化和动脉稳定.
皮埃佐1在血管发育中的作用在斑马鱼和哺乳动物之间功能上是保留的.

关键词:

这是一个Piezo1.动脉稳定动脉稳定壁画细胞的壁画.血管光滑肌肉的肌肉.斑马鱼是一种斑马鱼.

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