Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

<i>Oryza sativa</i> heavy metal ATPase (<i>OsHMA</i>) transporter-mediated cadmium uptake in rice: functional insights for low-Cd breeding.

Food chemistry. Molecular sciences·2026
Same author

Navigating a crowded developing brain leaves neurons with broken DNA.

Nature·2026
Same author

A critical initialization for biological neural networks.

Nature·2026
Same author

Duplex-Indel: a Snakemake pipeline for somatic Indel calling in Tn5 transposase-based duplex sequencing data.

Bioinformatics (Oxford, England)·2026
Same author

Somatic cancer variants enriched in Alzheimer's disease microglia-like cells drive inflammatory and proliferative states.

Cell·2026
Same author

DrPitA-mediated enrichment of intracellular manganese and phosphate contributes to oxidative stress resistance of <i>Deinococcus radiodurans</i>.

Applied and environmental microbiology·2026
Same journal

Layered social competition coordinates reproductive hierarchy formation in ants.

bioRxiv : the preprint server for biology·2026
Same journal

Combination epigenetic-targeted therapy increases the immunogenicity of poorly immunogenic sarcomas.

bioRxiv : the preprint server for biology·2026
Same journal

Loss of LanC-like proteins delays post-injury regeneration of aging skeletal muscles.

bioRxiv : the preprint server for biology·2026
Same journal

Integrative Transfer Network: Deep Transfer Learning Across Populations and Prediction Targets.

bioRxiv : the preprint server for biology·2026
Same journal

Confidence-supported label-free metabolic imaging with FPhaS phase autofluorescence microscopy.

bioRxiv : the preprint server for biology·2026
Same journal

Sequence-encoded autoinhibition couples mRNA decapping activity to phase separation.

bioRxiv : the preprint server for biology·2026
查看所有相关文章

相关实验视频

Updated: Jun 23, 2025

Examining Local Network Processing using Multi-contact Laminar Electrode Recording
13:40

Examining Local Network Processing using Multi-contact Laminar Electrode Recording

Published on: September 8, 2011

12.8K

空间单细胞分析解码皮质层和区域规范.

Xuyu Qian1,2,3, Kyle Coleman4,3, Shunzhou Jiang4,3

  • 1Division of Genetics and Genomics, Department of Pediatrics, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA.

bioRxiv : the preprint server for biology
|June 25, 2024
PubMed
概括
此摘要是机器生成的。

这项研究使用空间单细胞分析绘制了人类胎儿大脑发育的地图,揭示了早期层形成和视觉皮层中利的分子边界. 这挑战了梯度模型,并突出了大脑发育中的空间背景.

更多相关视频

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex
09:55

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Published on: September 5, 2018

8.4K
Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging
11:24

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Published on: December 12, 2012

13.7K

相关实验视频

Last Updated: Jun 23, 2025

Examining Local Network Processing using Multi-contact Laminar Electrode Recording
13:40

Examining Local Network Processing using Multi-contact Laminar Electrode Recording

Published on: September 8, 2011

12.8K
Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex
09:55

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Published on: September 5, 2018

8.4K
Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging
11:24

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Published on: December 12, 2012

13.7K

科学领域:

  • 神经科学是一个神经科学.
  • 发育生物学 发展生物学
  • 基因组学就是基因组学.

背景情况:

  • 人类大脑皮质对于认知至关重要,有六层和专门区域,由分子和结构组织定义.
  • 现有的单细胞转录组学失去了空间上下文,限制了对人类皮质发育的理解.
  • 空间关系对于皮质层和区域的分子规范至关重要.

研究的目的:

  • 检查人类胎儿皮质的分子,细胞和细胞架构的发展,以空间分辨的单细胞分辨率.
  • 在多个区域和时间点创建一个广泛的人类皮质发育的空间地图.
  • 调查皮层区域化和层形成背后的分子机制.

主要方法:

  • 利用多重错误强大的光在现场杂交 (MERFISH) 进行空间转录学.
  • 采用基于深度学习的细胞细分来进行增强分析.
  • 集成的MERFISH与单核RNA测序和在位整体转录组学.

主要成果:

  • 在八个皮质区域和四个妊娠时间点 (第二和第三个三个月) 建立了1600万个单细胞的空间地图.
  • 观察到六层结构在怀孕中期早期建立,这是在细胞架构层出现之前.
  • 在妊娠20周发现了初级 (V1) 和二级 (V2) 视觉皮层之间的利分子边界,其特点是神经元亚型规范的突然二进制转移,挑战了连续梯度模型.
  • 在V1特定的4层神经元中确定了突触发生的早期上调,这表明它在离散边界形成中的作用.

结论:

  • 空间关系对于皮质层和区域的分子规范至关重要.
  • 这些发现挑战了连续形态基因梯度仅仅决定妊娠中期皮质区域化的观念.
  • 这项工作提供了有价值的资源,并为未来的人类大脑发育地图建立了一个空间单细胞分析范式.