原始内皮支持血统的可塑性,使调节性发展成为可能
Madeleine Linneberg-Agerholm1, Annika Charlotte Sell1, Alba Redó-Riveiro1
1Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), University of Copenhagen, 2200 Copenhagen N, Denmark.
Cell
|June 25, 2024
概括
原始内皮 (PrE) 可以再生一个完整的哺乳动物胚囊,显示出发育可塑性. 这一发现揭示了 PrE
科学领域:
- 发育生物学
- 干细胞生物学
- 表观遗传学
背景情况:
- 哺乳动物的芽细胞形成需要 trofhectoderm 规范和内部细胞质量分化为表皮质和原始内皮质 (PrE).
- 胚胎具有发育可塑性的窗口,允许细胞命运重定向.
- 在胚胎细胞再生和可塑性中PrE的作用尚不完全理解.
研究的目的:
- 研究哺乳动物胚胎细胞形成中的原始内皮 (PrE) 的再生能力.
- 在体外识别和描述具有胚胎细胞形成潜力的干细胞群.
- 阐明抑制PrE承诺和维持发育可塑性的分子机制.
主要方法:
- 对早期哺乳动物胚胎进行实验操作.
- 在体外培养干细胞群.
- 对基因表达,转录因子活性和增强剂的分析.
- 抑制JAK/STAT信号通路和OCT4表达的研究.
主要成果:
- 单独的原始内皮 (PrE) 足以再生一个完整的胚胎细胞并支持植入后的发育.
- 在实验室中发现了一种类似PrE的群体,具有胚胎和胚胎外的效能,能够形成基于干细胞的胚胎模型 (胚胎).
- JAK/STAT信号,OCT4和持续的多能转录因子通过保持允许的增强剂环境来抑制 PrE 承诺.
结论:
- 原始内皮 (PrE) 具有显著的再生潜力,能够形成完整的胚囊.
- 持续的转录因子活性对于维持调节性发育中的可塑性至关重要.
- 在胚胎发育中,PrE起着至关重要的作用,特别是在乱的条件下,它是状体形成的关键.
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