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Updated: Jun 23, 2025

Using the E1A Minigene Tool to Study mRNA Splicing Changes
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用dCasRx-RBM2525对内源性外子拼接进行有效,特异和组合控制

Jack Daiyang Li1, Mikko Taipale1, Benjamin J Blencowe1

  • 1Donnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.

Molecular cell
|June 25, 2024
PubMed
概括

研究人员开发了一种基于CRISPR的新型系统dCasRx-RBM25,用于精确控制基因拼接. 这种强大的工具有效地准和修改内生替代外原体,为功能基因组学和基于拼接的疗法开辟了新的途径.

关键词:
克里斯普尔-卡斯连接屏幕在RNA结合蛋白质中的RNA结合蛋白.替代性拼接是一种替代性的拼接.失活的RfxCas13d (dCasRx) - 拼接因子在内源性外基子向上.拼接异形-分辨率功能基因组学

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