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Updated: Jun 27, 2026

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques
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使用干血斑点通过ELISA定量检测抗SARS-CoV-2IgG抗体:一个验证研究

Michelle Ylade1, Asma Binte Aziz2, Jedas Veronica Daag1

  • 1Institute of Child Health and Human Development, National Institutes of Health, University of the Philippines-Manila, Manila, Philippines.

The American journal of tropical medicine and hygiene
|June 25, 2024
PubMed
概括

干血斑 (DBS) 为SARS-CoV-2 IgG抗体检测提供了可行的血清替代品. 这种方法表现出高精度,简化了在资源有限的环境中大规模的血清检测.

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相关概念视频

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.