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评估用于检测和定量Cryptosporidium spp.的基于分子的方法. 在废水中

Oumaima Hachimi1, Rebecca Falender2, Gabriel Davis1

  • 1School of Chemical, Biological, and Environmental Engineering, Oregon State University, 116 Johnson Hall, Corvallis, OR 97331, USA.

The Science of the total environment
|June 25, 2024
PubMed
概括

对Cryptosporidium的废水监测对公共卫生至关重要. 这项研究比较了方法,发现离心法最适合卵巢恢复和珠子敲击用于DNA提取,而18SrRNA qPCR提供了优越的检测.

关键词:
在18S rRNA中.克里普托斯波里迪亚 spp.提取DNA 提取DNA的方法废水度 废水度 废水度废水监测 废水监测 废水监测 废水监测qPCRR 是一个很好的方法.

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Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...