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增强Pseudomonas细胞生长,用于从南极贝废弃物中生产中长链多基酸
Yueyue Zhou1, Xingyu Zhang1, Wenying Yu2
1Marine Economic Research Center, Donghai Academy, Ningbo University, Ningbo 315000, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo 315000, China; Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo 315000, China.
International journal of biological macromolecules
|June 25, 2024
概括
南极贝废弃物可以利用利用工程化Pseudomonas putida生产有价值的生物产品. 基因改造增强了细胞生长和从废物流中产生中长链多甲酸盐 (mcl-PHA).
科学领域:
- 生物技术是生物技术.
- 微生物工程 微生物工程
- 生物修复是一种生物修复.
背景情况:
- 南极贝废物 (AKSW) 丰富,但未得到充分利用.
- 伪虫 (Pseudomonas putida) 提供了从可再生资源合成生物产品的潜力.
- 在AKSW中的化物抑制了P. putida的生长,限制了其应用.
研究的目的:
- 为了研究P. putida对AKSW.的化物压力的转录反应.
- 为了更好地利用AKSW,对P. putida进行基因改造.
- 从AKSW.生产中链长的多基酸盐 (mcl-PHA).
主要方法:
- 在化物应激下对P. putida进行转录基因分析.
- 基于转录基因数据的P. putida的基因工程.
- 通过工程P. putida进行AKSW的发酵,用于mcl-PHA生物合成.
主要成果:
- 鉴定了P. putida对化物压力的转录反应.
- 改造的KT菌株+16840+03100显示细胞生长增加了33.7倍.
- 使用工程菌株从AKSW获得的mcl-PHA产量增加了40.3倍.
结论:
- 了解了P. putida对AKSW的化物压力的反应.
- 工程P. putida菌株是AKSW价值化的有效平台.
- 这项研究使从废物流中可持续生产mcl-PHA成为可能.
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