在Phyllostachys edulis中,CONSTANS-LIKE基因PeCOL13通过内部保留的替代拼接来调节开花
Hongjia Ma1, Jialong Pei1, Juan Zhuo1
1State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin' an 311300, China; Bamboo Industry Institute, Zhejiang A&F University, Lin' an 311300, China.
International journal of biological macromolecules
|June 25, 2024
概括
莫索竹的竹子可以使用.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 关于开花的规定 开花规则
背景情况:
- 木质竹子具有独特的,同步的开花模式,通常是死亡.
- 替代拼接 (AS) 是调节植物开花的关键机制.
- 在竹子开花过程中AS的作用在很大程度上仍未被探索.
研究的目的:
- 为了描述PeCOL13基因及其在moso竹 (Phyllostachys edulis) 中的异型.
- 研究PeCOL13异型体在调节开花时间方面的功能.
- 为了阐明竹子开花控制的基础分子机制.
主要方法:
- 基因克隆和异型鉴定通过替代拼接.
- 在不同生长阶段对PeCOL13异型的表达分析.
- 在大米 (Oryza sativa) 中进行过度表达研究,以评估开花时间的影响.
- 促进体结合测定和转录活性分析.
主要成果:
- 确定了两种PeCOL13异型,PeCOL13α和PeCOL13β,这些异型是由内子保留引起的.
- PeCOL13α的表达发生在植物和生殖阶段,而PeCOL13β显示出有限的生殖阶段表达.
- 过度表达PeCOL13α通过改变开花基因表达来延迟米头的发育,而PeCOL13β没有显著的影响.
- 这两种异型都与PeFT促进体结合;PeCOL13α抑制转录,但当同表达时,PeCOL13β会减弱这种抑制.
结论:
- PeCOL13α在调节莫索竹的开花时间方面发挥着至关重要的作用.
- 对PeCOL13的替代拼接产生具有独特调节功能的异型.
- PeCOL13异型之间的相互作用影响PeFT转录,提供了对竹子开花机制的见解.
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