通过MRM-IDA-EPI模式在生物矩阵中同时进行多个目标的法医毒理学选
Martina Franzin1, Rebecca Di Lenardo2, Rachele Ruoso2
1Institute for Maternal and Child Health, IRCCS "Burlo Garofolo", Trieste, Italy. martina.franzin@burlo.trieste.it.
Archives of toxicology
|June 25, 2024
概括
一种新的毒理学查方法可以在各种生物样本中准确识别751种药物和代谢物. 这种经过验证的方法为儿童中毒和法医病例提供了快速,敏感和特定的方法.
科学领域:
- 法医毒理学 法医毒理学
- 分析化学 分析化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 儿童中毒和过量服用死亡需要准确的药物鉴定.
- 目前的毒理学查方法可能缺乏全面的药物覆盖或速度.
- 经过验证的分析方法对于可靠的法医和临床毒理学至关重要.
研究的目的:
- 开发和验证一种新的多目标毒理学查方法.
- 将开发的方法的性能与液体染色学-质谱学/质谱学 (LC-MS/MS) 的性能进行比较.
- 评估该方法在现实世界儿童中毒和法医病例中的适用性.
主要方法:
- 开发一种使用多重反应监测信息依赖分析增强产品离子 (MRM-IDA-EPI) 的毒理学查方法.
- 在Kinetex Phenyl-Hexyl柱上使用梯度化进行染色学分离.
- 样本制备,包括对各种生物基质进行脱蛋白化和酶性水解.
主要成果:
- 该方法成功识别了751种药物和代谢物 (704种阳性,47种阴性).
- 在将查结果与所有测试矩阵的LC-MS/MS分析进行比较时,获得了最佳一致性 (100%).
- 该方法表现出高灵敏度,大多数分析物的切断值在最低测试度.
结论:
- 一种多目标,快速,敏感和特定的MRM-IDA-EPI查方法已成功开发和验证.
- 拟议的方法在各种毒理学领域具有广泛的适用性,包括儿童中毒和法医调查.
- 经过验证的方法为复杂的生物样本中的综合药物分析提供了可靠的工具.
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