相关实验视频

Updated: Jun 23, 2025

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

工程循环RNA用于分子和代谢重编程

Narendra Kumar Sharma¹, Pragya Dwivedi², Ravi Bhushan³

¹Department of Bioscience and Biotechnology, Banasthali Vidyapith (Deemed University), P.O. Banasthali Vidyapith Distt. Tonk, Rajasthan, 304 022, India. drnarendraks@gmail.com.

Functional & integrative genomics

|June 25, 2024

概括

循环RNAs (circRNAs) 为延长蛋白质表达提供了与信使RNA (mRNA) 的稳定替代品. 工程 circRNAs 增强了蛋白质生产,用于分子和代谢重编程的应用.

科学领域:

分子生物学分子生物学
生物技术是生物技术.
在RNA工程方面,RNA工程是非常重要的.

背景情况:

使者RNA (mRNA) 具有多样化的生物作用,但由于半衰期短和翻译效率低而受到限制.
循环RNAs (circRNAs) 是稳定的RNA分子,在真核细胞中通过背接合成.
合成和内源性circRNA可以编码蛋白质,将它们呈现为潜在的基因表达工具.

研究的目的:

总结外源性circRNA延长蛋白质表达持续时间的工程策略.
审查组装circRNAs和优化蛋白质生产的系统方法.
探索 circRNA 组件优化如何影响细胞重编程的翻译.

主要方法:

对外源性circRNA组件的工程方面的系统审查.
从工程 circRNAs 来影响蛋白质生产的因素的评估.
对circRNA组件 (矢量拓,UTR,IRES,aptamer) 的优化策略的分析.

主要成果:

工程方法可以显著延长来自circRNAs的蛋白质表达.
优化circRNA组件,如矢量拓和未翻译区域的影响蛋白质输出.
工程性体和内部核糖体进入点 (IRES) 影响翻译效率.

结论:

工程 circRNAs 为持续的蛋白质表达提供了一个强大的平台.
循环RNA工程能够精确控制蛋白质的生产,从而实现分子和代谢的重编程.
这项技术为调节细胞特征和治疗疾病提供了新的治疗可能性.

关键词:

克里斯普尔/Cas-13是什么意思克里斯普尔/Cas-9是什么意思循环RNAs是一种循环RNA.基因工程是一种基因工程.这里是IRES IRES.代谢重编程是一种代谢重编程.

更多相关视频

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Published on: February 24, 2023

相关概念视频

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

Methods of Nuclear Reprogramming

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

Introduction to Nuclear Reprogramming

Introduction to Nuclear Reprogramming

Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...

The Central Dogma

The Central Dogma

The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...

Somatic to iPS Cell Reprogramming

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...