Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Experimental RNAi02:15

Experimental RNAi

6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.8K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.8K
RNA Interference01:23

RNA Interference

26.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.0K
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

1.9K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
1.9K
The Central Dogma01:20

The Central Dogma

21.3K
The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
21.3K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.2K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Functional relevance of piRNA-PIWI axis in cancers: diagnostic and therapeutic avenues.

Cell cycle (Georgetown, Tex.)·2026
Same author

Molecular and pharmacological impacts of phytochemicals on the enoyl-acyl carrier protein reductase: potential therapeutic implications in tuberculosis.

Integrative biology : quantitative biosciences from nano to macro·2026
Same author

Azole resistance: patterns of amino acid substitutions in Candida sterol 14α-demethylase.

Antonie van Leeuwenhoek·2025
Same author

Role of RGD-binding Integrins in ovarian cancer progression, metastasis and response to therapy.

Integrative biology : quantitative biosciences from nano to macro·2025
Same author

GTF2I acts as a novel tumor suppressor transcription factor and shows Favorable prognosis in renal cancer.

Integrative biology : quantitative biosciences from nano to macro·2025
Same author

Acute Hypobaric Hypoxia Causes Alterations in Acetylcholine-Mediated Signaling Through Varying Expression of Muscarinic Receptors in the Prefrontal Cortex and Cerebellum of Rats' Brain.

High altitude medicine & biology·2024

相关实验视频

Updated: Jun 23, 2025

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

259

工程循环RNA用于分子和代谢重编程.

Narendra Kumar Sharma1, Pragya Dwivedi2, Ravi Bhushan3

  • 1Department of Bioscience and Biotechnology, Banasthali Vidyapith (Deemed University), P.O. Banasthali Vidyapith Distt. Tonk, Rajasthan, 304 022, India. drnarendraks@gmail.com.

Functional & integrative genomics
|June 25, 2024
PubMed
概括

循环RNAs (circRNAs) 为延长蛋白质表达提供了与信使RNA (mRNA) 的稳定替代品. 工程 circRNAs 增强了蛋白质生产,用于分子和代谢重编程的应用.

关键词:
克里斯普尔/Cas-13是什么意思克里斯普尔/Cas-9是什么意思循环RNAs是一种循环RNA.基因工程是一种基因工程.这里是IRES IRES.代谢重编程是一种代谢重编程.

更多相关视频

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

1.4K
Rapid Development of Cell State Identification Circuits with Poly-Transfection
09:21

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Published on: February 24, 2023

1.5K

相关实验视频

Last Updated: Jun 23, 2025

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

259
In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

1.4K
Rapid Development of Cell State Identification Circuits with Poly-Transfection
09:21

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Published on: February 24, 2023

1.5K

科学领域:

  • 分子生物学分子生物学
  • 生物技术是生物技术.
  • 在RNA工程方面,RNA工程是非常重要的.

背景情况:

  • 使者RNA (mRNA) 具有多样化的生物作用,但由于半衰期短和翻译效率低而受到限制.
  • 循环RNAs (circRNAs) 是稳定的RNA分子,在真核细胞中通过背接合成.
  • 合成和内源性circRNA可以编码蛋白质,将它们呈现为潜在的基因表达工具.

研究的目的:

  • 总结外源性circRNA延长蛋白质表达持续时间的工程策略.
  • 审查组装circRNAs和优化蛋白质生产的系统方法.
  • 探索 circRNA 组件优化如何影响细胞重编程的翻译.

主要方法:

  • 对外源性circRNA组件的工程方面的系统审查.
  • 从工程 circRNAs 来影响蛋白质生产的因素的评估.
  • 对circRNA组件 (矢量拓,UTR,IRES,aptamer) 的优化策略的分析.

主要成果:

  • 工程方法可以显著延长来自circRNAs的蛋白质表达.
  • 优化circRNA组件,如矢量拓和未翻译区域的影响蛋白质输出.
  • 工程性体和内部核糖体进入点 (IRES) 影响翻译效率.

结论:

  • 工程 circRNAs 为持续的蛋白质表达提供了一个强大的平台.
  • 循环RNA工程能够精确控制蛋白质的生产,从而实现分子和代谢的重编程.
  • 这项技术为调节细胞特征和治疗疾病提供了新的治疗可能性.