在无烟烟草产品中涉及的微生物社区和功能:一种元基因组方法
Mohammad Sajid1, Upma Sharma1, Sonal Srivastava1
1Division of Molecular Genetics and Biochemistry, Molecular Biology Group, ICMR-National Institute of Cancer Prevention and Research, Noida, India.
Applied microbiology and biotechnology
|June 25, 2024
概括
无烟烟草产品含有微生物,可以增强导致癌症的烟草特异性亚胺 (TSNA) 的合成. 这项研究确定了这些微生物及其功能,有助于无烟烟草戒烟战略.
科学领域:
- 微生物学 微生物学
- 毒理学 毒理学 毒理学
- 公共卫生 公共卫生
背景情况:
- 无烟烟草产品 (STP) 与口腔癌和病理有关.
- 致癌化合物,如烟草特有的尼托胺 (TSNA) 驱动STP相关的癌症.
- 在STP中,微生物代谢活动在TSNA合成中的作用在很大程度上尚未被探索.
研究的目的:
- 在印度STP中全面识别微生物基因和功能.
- 研究微生物活动与STP中有害化学物质水平之间的关系.
- 为制定有效的无烟烟草戒烟战略提供见解.
主要方法:
- 枪支元基因组测序被用来分析印度各种STP中的微生物群落.
- 进行化学分析以量化尼古丁,TSNA,真菌毒素和有毒金属.
- 确定了微生物的功能基因,特别是那些参与代谢和抗生素耐药性的基因.
主要成果:
- 在STP中占主导地位的微生物系是Actinobacteria,Proteobacteria和Firmicutes.
- 与代谢和抗生素耐药性相关的功能基因在STP微生物群中普遍存在.
- 在Mainpuri Kapoori中发现了高度的 ochratoxin-A,而在Moist-snuff,Mainpuri Kapoori和Gudakhu产品中发现了高度的TSNA和 (Zn).
结论:
- 这项研究揭示了与STP相关的微生物组的功能潜力,突出了其在TSNA合成中的作用.
- 抗生素耐药性基因和潜在病原体的存在强调了STP用户的健康风险.
- 这些发现支持针对无烟烟草的有针对性的干预措施和戒烟计划的制定.
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