高和正常葡萄糖适应中的TLR3介导的星细胞反应由甲胺不同的调节
Vladislav O Gorbatenko1, Sergey V Goriainov2, Valentina A Babenko3
1Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 119234, Moscow, Russia.
Cell biochemistry and biophysics
|June 25, 2024
概括
高葡萄糖会改变天体细胞对托尔类受体3 (TLR3) 激活的反应,影响炎症性细胞因子和氧利平的产生. 在这种高葡萄糖模型中,甲胺显示出抗炎潜力.
科学领域:
- 神经免疫学 神经免疫学
- 代谢性疾病研究研究.
- 细胞免疫学 细胞免疫学
背景情况:
- 收费类受体3 (TLR3) 是基质细胞上发现的关键的先天性免疫受体.
- 高血糖症显著影响炎症反应,需要在细胞模型中进行研究.
- 星球细胞在神经炎症中起着至关重要的作用,并受到代谢变化的影响.
研究的目的:
- 为了研究适应高葡萄糖 (HG) 条件的天体细胞的TLR3介导反应.
- 评估HG在TLR3激活后对细胞代谢和炎症媒介产生的影响.
- 评估甲福明在适应HG的星球细胞中的潜在抗炎作用.
主要方法:
- 星球细胞在正常 (NG) 和高葡萄糖 (HG) 条件下进行培养.
- 细胞被用多氨酸:多乙酸 (Poly(I:C)) 刺激,以激活TLR3.
- 使用海马XFp测量了新陈代谢概况 (ECAR,OCR).
- 用UPLC-MS/MS和其他测试分析了细胞因子,氧利平和信号通路激活.
主要成果:
- 高葡萄糖适应改变了基础ECAR和OCR,并减少了Poly (I:C) 对OCR的影响.
- 在HG条件下的多I:C刺激导致IL-1β和IL-10的减少,但增加了IL-6和iNOS的释放.
- HG适应降低了Poly (I:C) 诱导的氧利平合成,并没有影响p38,ERK,STAT3或ROS通路.
- 甲胺治疗减少了HG星球细胞中Poly (I:C) 刺激的细胞因子和氧利平的产生.
结论:
- 适应高葡萄糖的星细胞对TLR3激活的敏感性发生变化,影响炎症媒介的形状.
- 甲福明通过减轻这些高血糖引起的天体细胞反应变化,显示出抗炎性质.
- 这些发现突出了在糖尿病患者中管理神经炎症的潜在治疗策略.
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