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相关概念视频

RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

5.7K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
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相关实验视频

Updated: Jun 23, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

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基于对微生物群的测序分析.

Yishay Pinto1,2, Ami S Bhatt3,4

  • 1Department of Genetics, Stanford University, Stanford, CA, USA.

Nature reviews. Genetics
|June 25, 2024
PubMed
概括
此摘要是机器生成的。

最近的技术进步正在彻底改变微生物组研究. 科学家们现在可以更深入地了解微生物社区的组成和功能,并以前所未有的分辨率进行研究.

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Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
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Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

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Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
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Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Published on: May 2, 2018

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相关实验视频

Last Updated: Jun 23, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
11:22

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

27.9K
Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

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Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
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Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

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科学领域:

  • 微生物学 微生物学
  • 基因组学就是基因组学.
  • 环境科学 环境科学

背景情况:

  • 微生物组是多样化的社区,在农业,环境科学和人类健康方面发挥着重要作用.
  • 了解微生物组的功能和组成在各种科学学科中至关重要.

研究的目的:

  • 突出技术进步对微生物组研究的影响.
  • 为了强调对微生物组功能和组成的更好理解.

主要方法:

  • 使用高通量DNA和RNA测序.
  • 采用改进的长读测序技术.
  • 应用创新的计算分析方法.

主要成果:

  • 在基因组,转录和翻译层面深入了解微生物组.
  • 了解微生物组的功能和组成,超越物种层面.
  • 通过技术和分析进步促进微生物组研究.

结论:

  • 技术和分析方面的进步已经大大改善了微生物组研究.
  • 目前的方法可以更全面地了解微生物组的角色和动态.
  • 未来的研究可以以更高的分辨率探索微生物组功能.