与BM和脏相比,人类淋巴结中的先天性淋巴细胞的转录组多样性
Elaheh Hashemi1,2, Colleen McCarthy3, Sridhar Rao1,4,5
1Blood Research Institute, Versiti, Milwaukee, WI, USA.
Communications biology
|June 25, 2024
概括
这项研究揭示了人类淋巴结 (LNs) 中先天性淋巴细胞 (ILC) 的存在和发育阶段. 我们确定了新的ILC子集和监管网络,进步了对LN的免疫反应的理解.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 文字转录学 (Transcriptomics) 是一个学科.
背景情况:
- 天生的淋巴细胞 (ILC) 主要以它们在粘膜组织中的作用而闻名.
- 它们在人体排水淋巴结 (LNs) 中的存在和功能在很大程度上仍未被描述.
研究的目的:
- 综合描述人类腹部和胸部LNS中的ILCs的转录形状和发育阶段.
- 在LN中识别新的ILC子集和监管网络.
主要方法:
- 单细胞RNA测序47,287个CD127+ILCs来自人类LNs.
- 生物信息分析用于定义发育阶段,子集群和规则.
主要成果:
- 与骨髓 (BM) 或脏相比,人类LNs的CD127+ILC频率更高.
- 在LN中定义了独立的ILC发展阶段 (EILP,pILC) 和确定了天真的ILC (nILC).
- 在LN中特征了三个ILC1和四个ILC3子集群,揭示了不同的转录特征,包括热冲击蛋白编码基因.
- 确定了新的规则,例如BACH2用于ILC1和ATF用于ILC3.
结论:
- 这项研究提供了迄今为止人类LNs中ILCs最广泛的表征.
- 发现了以前未知的ILC发育途径和LNs中的子集.
- 提供了对人类淋巴系统内ILC介导免疫的关键见解.
更多相关视频
06:33Author Spotlight: Comprehensive Epigenetic Analysis for Investigating Human Cellular Plasticity and Environmental Adaptation Using Immunofluorescence Assays
Published on: June 28, 2024
506
14:43Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation
Published on: October 1, 2012
18.4K
相关概念视频
01:23Cells of the Adaptive Immune Response
979
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
979
01:28Diversity of Antigen Receptors
566
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
566