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异种雌激素及其与人类基因和细胞蛋白的相互作用:一个体内研究
Arathy V Warrier1, Manasa Vg1, Neetha R L1
1Cancer Research Program 6, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India.
Asian Pacific journal of cancer prevention : APJCP
|June 25, 2024
概括
暴露于异性雌激素,如双-A,与参与乳腺癌发展的基因有关. 这些已识别的基因在乳腺癌中表达更高,这表明环境雌激素模仿在疾病中的作用.
科学领域:
- 环境毒理学环境毒理学
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 雌激素在乳腺癌的发展中起着关键作用.
- 每天暴露于环境雌激素模仿剂 (外雌激素) 是很常见的.
- 对与癌症相关的信号通路和基因相互作用的异性雌激素的影响尚未完全理解.
研究的目的:
- 为了确定异性雌激素的直接或间接结合伙伴.
- 为了研究暴露于雌激素化合物的基因表达变化.
- 探索异性雌激素在乳腺癌发病过程中的作用.
主要方法:
- 从比较毒基因组学数据库中收集了与四种雌激素相关的基因 (奥基,诺基,双-A,2,2-bis(4-基) -1,1,1-三乙烯).
- 利用Venny 2.1来识别异性雌激素之间共享的基因.
- 使用DAVID进行基因本体学和KEGG通路分析.
- 使用STITCH.构建了一个异性雌激素-蛋白相互作用网络.
- 通过UCSC Xena.使用微阵列数据集GSE5200和乳腺癌亚型分析基因表达.
主要成果:
- 确定了与所有四种研究的异性雌激素相互作用的13个基因.
- 途径分析揭示了癌症途径,化学致癌和雌激素信号的丰富.
- 异雌激素-蛋白相互作用网络的结果与数据库的发现进行了验证.
- 微阵列数据显示,在双A和诺尼尔治疗的MCF-7细胞中,13个基因的显著高表达.
- 这些基因主要以光线A或基底乳腺癌亚型表达.
结论:
- 与四种异性雌激素相关的基因主要与瘤发生途径有关.
- 这些与外雌激素相关的基因的表达在乳腺癌中升高.
- 这表明一个潜在的机制,通过环境雌激素模仿促进乳腺癌的发展.
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