在Streptococcus mutans中的SepM突变临床分离物和相关功能分析分析
Shanshan Liu1,2, Yidan Shao2, Zhenzhen Zhang3
1Department of Stomatology, The First Affiliated Hospital of Bengbu Medical College, 287 Chuang Huai Road, Bengbu, 233004, China.
BMC oral health
|June 25, 2024
概括
Streptococcus mutans sepM 基因的突变增强了它抑制 Streptococcus gordonii 生长的能力. 这些sepM突变增加了CSP-21的分裂,从而产生更强的抑制作用.
科学领域:
- 微生物学 微生物学
- 细菌病原体的产生
- 分子生物学分子生物学
背景情况:
- 突变性链球菌 (S. mutans) 导致牙变.
- 红斑杆菌 (S. gordonii) 抑制了S. mutans的生长.
- 在S. mutans中的SepM蛋白通过裂解CSP-21并激活ComDE系统来促进S. gordonii的抑制.
研究的目的:
- 在S. mutans临床分离物中研究sepM突变.
- 确定sepM突变在调节S. mutans与S. gordonii的相互作用中的作用.
主要方法:
- 将286种S. mutans菌株分为抑制性 (n=114) 和非抑制性 (n=172) 组.
- 执行了sepM基因的桑格测序.
- 分析了基因/蛋白质表达,净化了突变蛋白质,并评估了SepM-CSP-21的结合亲和力.
主要成果:
- 在抑制组中,C482T,G533A和G661A突变更频繁.
- 突变的SepM (G533A,G661A) 以pH取决的方式增加了与CSP-21的结合亲和力.
- 在具有G533A突变的隔离物中,SepM,化ComD和ComE的表达更高.
结论:
- 突变的sepM增强了S. mutans对S. gordonii的抑制.
- 由突变的SepM增加的CSP-21裂变有助于抑制作用.
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