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Conservative Site-specific Recombination and Phase Variation02:53

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Septins are protein filaments forming the cytoskeleton along with the microtubules, microfilaments, intermediate filaments, and other accessory proteins. In 1971 while studying the cell division cycle in mutant Saccharomyces cerevisiae Harwell et al. first identified the septin-related genes playing a crucial role in yeast cytokinesis. Fluorescence microscopy revealed that these proteins localize at the budding neck as rings. These ring-like proteins were then named Septins by John Pringle, and...
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相关实验视频

Updated: May 6, 2026

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
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在Streptococcus mutans中的SepM突变临床分离物和相关功能分析分析.

Shanshan Liu1,2, Yidan Shao2, Zhenzhen Zhang3

  • 1Department of Stomatology, The First Affiliated Hospital of Bengbu Medical College, 287 Chuang Huai Road, Bengbu, 233004, China.

BMC oral health
|June 25, 2024
PubMed
概括

Streptococcus mutans sepM 基因的突变增强了它抑制 Streptococcus gordonii 生长的能力. 这些sepM突变增加了CSP-21的分裂,从而产生更强的抑制作用.

关键词:
卡里斯 卡里斯 卡里斯 卡里斯表达方式表达方式表达基因多态化是基因的多态化.七月 七月 七月 七月

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科学领域:

  • 微生物学 微生物学
  • 细菌病原体的产生
  • 分子生物学分子生物学

背景情况:

  • 突变性链球菌 (S. mutans) 导致牙变.
  • 红斑杆菌 (S. gordonii) 抑制了S. mutans的生长.
  • 在S. mutans中的SepM蛋白通过裂解CSP-21并激活ComDE系统来促进S. gordonii的抑制.

研究的目的:

  • 在S. mutans临床分离物中研究sepM突变.
  • 确定sepM突变在调节S. mutans与S. gordonii的相互作用中的作用.

主要方法:

  • 将286种S. mutans菌株分为抑制性 (n=114) 和非抑制性 (n=172) 组.
  • 执行了sepM基因的桑格测序.
  • 分析了基因/蛋白质表达,净化了突变蛋白质,并评估了SepM-CSP-21的结合亲和力.

主要成果:

  • 在抑制组中,C482T,G533A和G661A突变更频繁.
  • 突变的SepM (G533A,G661A) 以pH取决的方式增加了与CSP-21的结合亲和力.
  • 在具有G533A突变的隔离物中,SepM,化ComD和ComE的表达更高.

结论:

  • 突变的sepM增强了S. mutans对S. gordonii的抑制.
  • 由突变的SepM增加的CSP-21裂变有助于抑制作用.