在Corynebacterium glutamicum中通过路径工程和过程优化进行β-arbutin的新生态合成
Bin Zhang1,2, Kexin Gou3,4, Kexin Xu3,4
1College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China. zhangbin2919@163.com.
Biotechnology for biofuels and bioproducts
|June 25, 2024
概括
研究人员对Corynebacterium glutamicum进行了新的β-arbutin生物合成的工程,达到7.94g/L的产量标位. 这一突破为可持续的β-arbutin生产提供了一种高性能微生物菌株.
科学领域:
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
- 微生物生物技术 微生物生物技术
背景情况:
- β-阿布丁是一种植物衍生的基诺糖化物,具有宝贵的抗氧化,抗炎,抗微生物和抗癌性质.
- 它在制药和化品行业得到广泛应用.
- 目前高性能菌株的局限性阻碍了有效的生物基β-arbutin生产.
研究的目的:
- 在Corynebacterium glutamicum中建立β-arbutin的新生物合成途径.
- 通过代谢工程策略来提高β-arbutin生产标位.
- 开发一种强大的微生物底盘,用于可持续的β-arbutin生物合成.
主要方法:
- 引入编码子优化的基因 (ubiC,MNX1,AS) 来构建β-arbutin生物合成途径.
- 竞争的代谢途径 (csm,trpE) 的非激活,以重定向代谢流量.
- 优化上游代谢途径和补充UDP-葡萄糖.
主要成果:
- 在C. glutamicum ATCC13032.2.中成功确定了de novoβ-arbutin生物合成途径.
- 工程菌株C. glutamicum AR11实现了最终β-arbutin产量标位为7.94g/L.
- 优化的发酵条件和代谢修改显著提高了生产.
结论:
- 这项研究报告了C. glutamicum中第一个成功的de novo生产β-arbutin.
- 经过工程改造的C. glutamicum菌株作为未来β-arbutin生物合成的有希望的底盘.
- 这项工作为 β-arbutin 的可持续和可扩展的生物基生产铺平了道路.
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