人类静脉血液和毛细血管血液中血清25氧维生素D水平的两个检测方法之间的相关性和一致性
Yutong Xing1, Kaixi Wang1, Xinyu Ma1
1Department of Paediatrics, The Second Hospital Affiliated to Hebei Medical University, Shijiazhuang, China.
Frontiers in nutrition
|June 26, 2024
概括
这项研究发现,对25-氧维生素D [25(OH) D]的毛细血管血液检测与静脉血液检测有很好的相关性. 标准化毛细血管检测为监测维生素D水平提供了可靠的替代方案.
科学领域:
- 临床生物化学 临床生物化学
- 营养科学 营养科学
- 分析化学 分析化学
背景情况:
- 准确测量25-氧维生素D [25(OH) D]对于评估维生素D状态至关重要.
- 静脉血液采样是标准的,但毛细血管血液提供了一个不那么侵入性的替代方案.
- 评估毛细血管和静脉25(OH) D测量之间的一致性对于临床实用性至关重要.
研究的目的:
- 评估同时收集的毛细血管和静脉血中25...OH) D水平之间的相关性和一致性.
- 建立转换方程,将毛细血管25(OH) D测量转换为静脉等价物.
- 确定两种样本类型和检测方法之间的临床诊断协议.
主要方法:
- 42名健康成年人在12个月内接受了不同的维生素D3补充方案.
- 在多个时间点同时采集了毛细血管和静脉血液样本.
- 使用化学发光免疫测试 (CLIA) 和液态染色体质谱法 (LC-MS) 测量了25(OH) D水平.
- 统计分析包括皮尔森相关性,线性回归,布兰德-阿尔特曼,卡帕分析和ROC曲线.
主要成果:
- 在静脉和毛细血管25(OH) D水平之间观察到强烈的线性相关性 (R2 = 0.625).
- 得到的公式是:静脉25(OH) D = 1.105 *毛细血管25(OH) D - 7.532.
- 在静脉和纠正的毛细血管之间发现了良好的一致性 (Kappa = 0.75),用于临床诊断.
- 毛细血管血液测量结果显示,与静脉样本相比,平均值更高 (LC-MS: 52.03 与 45.32 nmol/L 相比).
结论:
- 使用CLIA的标准化毛细血管血液检测表明与静脉血液测量25...OH) D的良好一致.
- 毛细血管血液25(OH) D测量,当调整时,具有很高的临床预测价值.
- 毛细血管血液采样是一种可行且可能更方便的25(OH) D检测方法.
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