在创建LBP和基于Limosilactobacillus fermentumU-21的后生菌中使用omics技术
Maya V Odorskaya1, Dilara A Mavletova1, Andrey A Nesterov1,2
1Laboratory of Bacterial Genetics, Vavilov Institute of General Genetics Russian Academy of Sciences, Moscow, Russia.
Frontiers in microbiology
|June 26, 2024
概括
这项研究探讨了针对神经炎症的新型活生物疗法 (LBPs) 和后生物药的Limosilactobacillus fermentum U-21. 它确定了GABA和尼亚等关键蛋白质和代谢物,展示了神经退行性疾病治疗的潜力.
科学领域:
- 微生物学和生物技术
- 神经科学是一个神经科学.
- 药理学 药理学是指药理学的学科.
背景情况:
- 制药生物制剂的发展,包括活生物疗法 (LBPs) 和后生物制剂,依赖于了解细菌代谢物及其机制.
- 神经炎症是神经退行性疾病的关键因素,因此需要新的治疗策略.
研究的目的:
- 研究Limosilactobacillus fermentum U-21作为创新的LBP和针对神经炎症过程的后生菌的来源的潜力.
- 鉴定和描述由L. fermentum U-21.21产生的药理活性蛋白质和代谢物.
主要方法:
- 我们使用了omics技术 (基因组学,蛋白质组学,代谢组学) 来分析L. fermentum U-21.
- 蛋白质组分析确定了参与分聚酶,抗氧化剂和免疫调节活动的关键蛋白质.
- 代谢分析确定了生物活性化合物,如GABA,尼亚,aucubin和scylo-inositol.
主要成果:
- 蛋白质组分析揭示了诸如ClpL,DnaK,GrpE,硫素还原酶,LysM和NlpC/P60域含有蛋白质等蛋白质.
- 代谢分析检测到GABA,尼亚,奥库和西洛-伊诺西托,具有证明的抗神经炎症作用.
- GABA稳定神经元活动,尼亚调节细胞因子的产生,aucubin表现出抗氧化特性,而syllo-inositol作为化学伴侣.
结论:
- Limosilactobacillus fermentum U-21产生具有显著免疫调节,抗神经炎症和神经调节活性的生物活性物质.
- 这些发现支持对神经退行性疾病的L. fermentum U-21衍生药生物的开发.
- 已识别的化合物和蛋白质为神经系统疾病提供了潜在的治疗点.
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