Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

The Role of Actin and Myosin in Non-muscle Cells01:10

The Role of Actin and Myosin in Non-muscle Cells

3.5K
Actin and myosin or actomyosin filaments also play a significant role in cells other than those involved in muscle contraction (which occurs within the sarcomere of muscle cells). The mechanism of non-muscle cell contractile bundles was first observed in Dictyostelium and Acanthamoeba. In non-muscle cells, two bundles are commonly found: stress fibers and actomyosin adherence belts. These contractile bundles are smaller and less organized than the ones found in muscle cells. They  are held...
3.5K
Smooth Muscle Contraction01:25

Smooth Muscle Contraction

2.7K
Smooth muscle contraction is a complex process vital for various bodily functions, from maintaining blood vessel tension to facilitating the movement of food through the digestive tract. Unlike striated muscles, smooth muscle contraction begins more slowly and lasts longer.
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
2.7K
Introduction to Actin01:26

Introduction to Actin

5.1K
Actin is a highly conserved cytoskeletal protein found abundantly in eukaryotic cells. It constitutes 10% weight of the total cellular protein in muscle cells, while in non-muscle cells, it is lower and makes up around 1–5 percent of the total cell protein. Actin found in the unicellular amoebae and complex multicellular animals is around 80% similar, demonstrating their conservation over a billion years of evolution.  Actin coding genes are conserved within species and across...
5.1K
Structure and Organization of Smooth Muscles01:13

Structure and Organization of Smooth Muscles

5.6K
Smooth muscle tissue is a type of muscle tissue that can be found lining various vital organs in the human body, including the lungs, blood vessels, digestive tract, and respiratory tract. This type of tissue is responsible for regulating the movements of these organs, playing crucial roles in the functioning of various systems, including the vascular, digestive, respiratory, and urinary systems.
Structure of smooth muscle cell
Smooth muscle cells are spindle-shaped with tapering ends and a...
5.6K
Actin Polymerization and Cell Motility01:13

Actin Polymerization and Cell Motility

5.2K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
5.2K
Role of Myosin in Cell Migration01:18

Role of Myosin in Cell Migration

2.3K
Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II  is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
2.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

XGBoost Model With CMR Features for Prognostic Assessment in Patients With ST-Segment Elevation Myocardial Infarction.

JACC. Asia·2026
Same author

Adherence to Survivorship Care Visits in Patients With Cervical Cancer in Botswana.

JCO global oncology·2026
Same author

Neutralization of reactive oxygen species with cobinamide augments aortic dissections and rupture in BAPN-administered mice.

American journal of physiology. Heart and circulatory physiology·2026
Same author

Branched endovascular aortic arch repair to treat intermittent cerebral malperfusion syndromes after type A aortic dissection repair.

JTCVS structural and endovascular·2026
Same author

Hybrid aortic arch repair for recoarctation and arch aneurysm with concomitant multivalvular surgery.

JTCVS structural and endovascular·2026
Same author

An intelligent wearable device based on electrical impedance tomography for dynamic and accurate monitoring of voiding function in patients with benign prostatic hyperplasia: protocol for a single-centre self-controlled diagnostic consistency study.

Translational andrology and urology·2026

相关实验视频

Updated: Jun 23, 2025

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro
08:28

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

Published on: February 15, 2022

3.7K

核光滑肌肉α-actin参与血管光滑肌肉细胞分化.

Callie S Kwartler1, Albert J Pedroza2, Anita Kaw1

  • 1Division of Medical Genetics, Department of Internal Medicine, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030.

Nature cardiovascular research
|June 26, 2024
PubMed
概括

核光滑肌肉α-actin (αSMA) 对于光滑肌肉细胞分化至关重要. 在ACTA2中的致病变体,特别是在阿金179中,破坏了这种核作用,导致血管疾病.

更多相关视频

Culturing and Manipulation of O9-1 Neural Crest Cells
08:32

Culturing and Manipulation of O9-1 Neural Crest Cells

Published on: October 9, 2018

10.1K
Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice
09:06

Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice

Published on: February 20, 2019

8.3K

相关实验视频

Last Updated: Jun 23, 2025

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro
08:28

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

Published on: February 15, 2022

3.7K
Culturing and Manipulation of O9-1 Neural Crest Cells
08:32

Culturing and Manipulation of O9-1 Neural Crest Cells

Published on: October 9, 2018

10.1K
Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice
09:06

Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice

Published on: February 20, 2019

8.3K

科学领域:

  • 遗传学 是一个遗传学.
  • 细胞生物学 细胞生物学
  • 心血管研究研究心血管研究

背景情况:

  • 编码光滑肌肉α-actin (αSMA) 的ACTA2中的Missense变异与胸前大动脉疾病有关.
  • 在阿尔金宁179 (R179) 的特定变体导致平滑肌肉功能障碍综合征 (SMDS),呈现儿童血管疾病.

研究的目的:

  • 研究核αSMA在光滑肌肉细胞 (SMC) 分化中的作用.
  • 确定ACTA2 R179变体对αSMA核定位和SMC功能的影响.

主要方法:

  • 免疫光显微镜用于评估αSMA在野生类型 (WT) 和突变细胞中的核定位.
  • 在实验室和体内分化测试中使用来自Acta2突变小鼠的初级SMC.
  • 对来自患者的诱导多能干细胞的染色质可访问性和分化的分析.
  • 患者大动脉组织的单细胞转录组分析.

主要成果:

  • WT αSMA在分化SMC中定位到核中,并与染色体重塑复合体相关联.
  • 该ACTA2 p.R179变种表现出αSMA.的核定位减少.
  • 在Acta2突变的SMC中,分化功能受损,染色质可访问性发生变化.
  • 来自患者的细胞和组织揭示了SMC分化失败和SMC可塑性增加.

结论:

  • 核αSMA在SMC分化和基因调节中发挥着关键作用.
  • 致病ACTA2 R179变种破坏αSMA核功能,导致SMC功能障碍和血管疾病的发展.