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开发一款自动供电的数字LAMP微流体芯片 (SP-dChip),用于检测新出现的病毒

Tom Kasputis¹, Po-Chen Yeh², Li Liu³

¹Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA, USA.

|June 26, 2024

概括

本研究引入了一种自动供电的数字循环介导同热放大 (dLAMP) 微流体芯片,用于快速,定量检测核酸. 这种创新的设备可以对像寨卡病毒 (ZIKV) 这样的新兴病毒进行敏感的临床诊断.

科学领域:

生物技术是生物技术.
微流体学微流体学
分子诊断学分子诊断学

背景情况:

护理点 (POC) 诊断对于快速检测新出现的传染病至关重要.
当前的POC设备往往缺乏灵敏度和可靠的定量读数.
需要改进的POC技术用于传染病监测.

研究的目的:

开发一个自动供电的数字循环介导同热放大 (dLAMP) 微流体芯片 (SP-dChip).
为了使核酸的快速,定量和现场检测.
为了证明SP-dChip在检测寨卡病毒 (ZIKV) RNA方面的能力.

主要方法:

一种新的真空肺设计,用于将样品被动数字化成纳米升井.
与dLAMP微流体芯片集成反转录循环介导的同热放大 (RT-LAMP).
装载LAMP试验,被动数字化和以石油为基础的井区分,用于个别反应监测.

主要成果:

在没有外部电源或送的情况下,SP-dChip实现了快速可靠的ZIKV RNA检测.
该系统在一个小时内显示的检测极限低至每μL100个副本.
高通量分析是通过被动样本数字化到单个井的实现的.

结论:

SP-dChip提供了一种新的解决方案,用于负担得起,便携式和定量POC诊断.
这项技术可以显著提高在护理点检测新兴病毒的能力.
自动供电的数字微流体方法解决了当前POC诊断设备的局限性.

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