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相关概念视频

Combinatorial Gene Control02:33

Combinatorial Gene Control

8.3K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.3K
Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.3K
Structure of a Gene01:30

Structure of a Gene

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A gene is the fundamental unit of heredity. Every individual has two copies of each gene, one inherited from each parent. Although most people contain the same genes, there is a small fraction that is slightly different amongst people. A gene with a small difference in its sequence of DNA bases forms different alleles, contributing to different phenotypes.
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...
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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K
What is Gene Expression?01:42

What is Gene Expression?

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Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
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相关实验视频

Updated: Jun 23, 2025

Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

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器官特异性基因表达控制使用基于DNA原形的纳米设备.

Yuxiang Liu1, Ruixuan Wang1, Qimingxing Chen1

  • 1School of Life Science and Technology, ShanghaiTech University, Shanghai 201210 People's Republic of China.

Nano letters
|June 26, 2024
PubMed
概括

研究人员开发了在基于脂质的纳米颗粒 (LNP) 中封装的强大的DNA原始体纳米设备,以增强器官特异性基因传递. 这一突破改善了小鼠器官中信使RNA (mRNA) 和小干扰RNA (siRNA) 的功能.

关键词:
化电磁波是一种冷电磁波.基因原始的DNA原始化在LNP中使用LNP.基因表达的基因表达方式

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Designing a Bio-responsive Robot from DNA Origami
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Designing a Bio-responsive Robot from DNA Origami

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DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications
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DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

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相关实验视频

Last Updated: Jun 23, 2025

Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

14.6K
Designing a Bio-responsive Robot from DNA Origami
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Designing a Bio-responsive Robot from DNA Origami

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DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications
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DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

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科学领域:

  • 合成生物学 合成生物学
  • 纳米技术纳米技术
  • 基因输送 基因输送 基因输送

背景情况:

  • 实现特定器官的纳米设备功能是合成生物学的一个关键目标.
  • 基因组件使得纳米设备的构造能够实现有针对性的输送,但脆弱性和低向性限制了器官的输送.
  • 当前的方法在生理条件下保持纳米结构完整性方面面临挑战.

研究的目的:

  • 为了设计强大的DNA原形纳米设备,用于器官特定的基因传递.
  • 为了克服DNA纳米结构脆弱性和准的局限性.
  • 为了增强目标器官内的感兴趣基因 (GOI) 有效载荷的功能.

主要方法:

  • 坚固的DNA原始结构纳米结构的构建.
  • 在低pH条件下将DNA原始体封装成基于脂质的纳米粒子 (LNP).
  • 在小鼠器官中评估GOI (mRNA和siRNA) 的功能.
  • 低温电子显微镜 (Cryo-EM) 用于LNP结构分析.

主要成果:

  • 成功创建了DNA原形封装LNP,在恶劣条件下稳定.
  • 在小鼠器官中显示了mRNA和siRNA有效载荷的功能增加.
  • 确定了不同的LNP结构,有助于通过Cryo-EM增强有效载荷传送.

结论:

  • 用DNA原形封装的LNP为器官特异性基因传递提供了一个有希望的策略.
  • 开发的纳米设备提高了基因表达控制的有效性.
  • 这些发现为针对性基因疗法和合成生物学应用的未来进步提供了基础.