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基于酸盐膜的色度测量葡萄糖生物传感器及其在饮料中的葡萄糖检测中的应用,使用智能手机应用程序
Anastasia Skonta1, Myrto G Bellou1, Theodore E Matikas2
1Laboratory of Biotechnology, Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, Greece.
Biosensors
|June 26, 2024
概括
新的基托桑基生物传感器薄膜与葡萄糖氧化酶 (GOx) 固定,提供准确和稳定的葡萄糖检测. 这种颜色测量方法可用于智能手机,增强了食品和饮料的质量控制.
科学领域:
- 生物材料科学 生物材料科学
- 分析化学 分析化学
- 食品科学 食品科学 食品科学
背景情况:
- 生物传感器对于食品和饮料的质量控制越来越重要,因为它们的灵敏度,特异性和速度.
- 与智能手机应用程序集成的色度生物传感器提供了可访问的实时分析物检测.
- 酸盐 (CS) 和深度化溶剂 (DES) 为酶固定提供了一个有前途的矩阵.
研究的目的:
- 开发和优化用葡萄糖氧化酶 (GOx) 固定化基托桑膜,用于葡萄糖检测.
- 为了研究pH值和温度对膜内的酶活性的影响.
- 评估开发的生物传感器膜的稳定性,特异性和分析性能.
主要方法:
- 使用胆化物/尿素/甘油 (ChCl:U:Gly) 深度欧性溶剂制备奇托 (CS) 薄膜.
- 使用谷氨 (GA) 将葡萄糖氧化酶 (GOx) 固定在CS膜上.
- 优化GOx度和DES含量,然后对薄膜特性和酶活性进行表征.
主要成果:
- 优化的CS/GOx膜显示出高精度,对葡萄糖的特异性和在4°C下4周的稳定性.
- 在经过测试的pH和温度条件下,固定的GOx保持了显著的活动.
- 生物传感器膜的线性响应范围为0.1-0.8mM,检测极限 (LOD) 为33μM.
结论:
- 开发的奇托/葡萄糖氧化酶膜是稳定的,可重复使用的,并且对检测葡萄糖具有高度特异性.
- 生物传感器与智能手机颜色识别应用程序的兼容性使其能够在食品和饮料质量评估中实际使用.
- 这项技术提供了一种具有成本效益和高效的方法,用于现场估计葡萄糖度.
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