更新:通过分子检测和抗真菌管理进行疫情评估 - - 叙述审查
Silvia Ionescu1, Ionut Luchian2, Costin Damian1
1Department of Preventive Medicine and Interdisciplinarity (IX), Microbiology, Faculty of Medicine, "Grigore T. Popa" University of Medicine and Pharmacy, 700115 Iasi, Romania.
Current issues in molecular biology
|June 26, 2024
概括
对于Candida auris爆发的分子检测方法是多样化的,从VITEK® 2到高级测序. 实时PCR测定对于快速,负担得起的诊断和抗真菌耐药基因检测至关重要.
科学领域:
- 医学真菌学 医学真菌学
- 传染性疾病 传染性疾病
- 分子诊断学 分子诊断学
背景情况:
- 黄是一种具有全球意义的真菌病原体,由于其抗真菌耐药性和高死亡率,世界卫生组织将其列为最优先的病原体之一.
- 已经更新了真菌病原体的分类,包括Nakaseomyces glabrata (原Candida glabrata) 和Pichia kudriavzevii (原Candida krusei).
- 了解Candida auris爆发的分子检测方法对于有效的控制和治疗至关重要.
研究的目的:
- 在过去五年中,审查和评估用于检测Candida auris爆发的分子诊断试验.
- 确定已发表的Candida auris疫情调查中使用的分子技术范围.
- 强调开发可访问的Candida auris诊断工具的重要性,包括检测抗真菌耐药性的工具.
主要方法:
- 在PubMed.中使用关键词如"Candida auris"",疫情"和"分子检测"进行了系统的文献搜索.
- 选择和分析了2019年至2024年间发表的23篇原创研究论文.
- 该研究审查了各种分子检测方法,包括VITEK® 2,MALDI TOF,下一代测序 (NGS),全基因组测序 (WGS) 和实时聚合酶链反应 (PCR).
主要成果:
- 广泛的分子检测方法已被用于全球的Candida auris爆发.
- 研究采用了从VITEK® 2等传统系统到NGS和WGS等先进的测序技术的各种技术.
- 检测抗真菌耐药基因,如FKS和ERG11,在几个研究中被确定,与治疗挑战相关.
- 在所有大陆都报告了Candida auris的爆发,这强调了其全球传播和环境持久性.
结论:
- 目前用于检测Candida auris爆发的多种分子测试,反映了诊断能力的进步.
- 开发和实施准确,负担得起的实时PCR测定对于广泛的实验室使用至关重要.
- 促进快速检测Candida auris及其耐药基因将使及时有效的医院管理计划成为可能.
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