在初级抗体缺乏症患者中,SARS-CoV-2疫苗接种后的适应性细胞反应
Sudhir Gupta1, Houfen Su1, Sudhanshu Agrawal1
1Program in Primary Immunodeficiencies, Division of Basic and Clinical Immunology, University of California at Irvine, Irvine, CA 92697, USA.
Pathogens (Basel, Switzerland)
|June 26, 2024
概括
在SARS-CoV-2疫苗接种后,原发性抗体缺乏症患者 (PADs) 显示出显著的B细胞变异,包括调控性B细胞 (Breg). 尽管有一些T细胞变化,但这些发现表明PAD患者可以获得有效的疫苗反应.
科学领域:
- 免疫学 免疫学 免疫学
- 疫苗学 疫苗学 疫苗学
- 传染性疾病 传染性疾病
背景情况:
- 天生的免疫错误 (IEI) 增加了SARS-CoV-2感染的风险.
- 主要抗体缺陷 (PADs) 显示出低于预期的严重COVID-19结果.
- 这表明疫苗在PAD患者中具有潜在的疗效,尽管抗体反应数据相互矛盾.
研究的目的:
- 在PAD患者中全面评估淋巴细胞群对SARS-CoV-2疫苗接种的反应.
- 研究T细胞,B细胞和调控性淋巴细胞子集的表型变化.
- 评估SARS-CoV-2特异性T细胞激活和细胞毒性T淋巴细胞 (CTL) 反应.
主要方法:
- 疫苗接种后对各种淋巴细胞子集 (T细胞,B细胞,调节性淋巴细胞) 的表型分析.
- 对T细胞激活标记物 (CD69,CD137) 和SARS-CoV-2四聚体+CD8 T细胞的流细胞计评估.
- 对T毛囊辅助细胞 (Tfh) 和调控性T毛囊细胞 (Tfr) 子集的评估.
主要成果:
- 在各种B细胞子集中观察到显著的变化,特别是调控性B细胞 (Breg).
- 在大多数T细胞子集 (CD4,CD8,TCM,TEM,TEMRA) 中检测到有限的变化.
- 还评估了SARS-CoV-2特异性T细胞激活和CD8 CTL反应.
结论:
- 在接种SARS-CoV-2疫苗后,PAD患者在B细胞种群中表现出显著的变化.
- 观察到的免疫细胞反应表明,大多数PAD患者对疫苗接种有有效的反应.
- 需要进一步的研究,以充分阐明IEI人群中疫苗诱导的免疫力.
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