对引起小牛乳腺炎的病原体产生不同的免疫反应
Anyaphat Srithanasuwan1,2, Noppason Pangprasit3, Raktham Mektrirat4,5
1Veterinary Science Unit, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.
非光性葡萄球菌 (NASM) 损害了牛中性粒细胞对乳腺炎的防御能力,与乳酸细菌 (LAB) 不同. 纳斯姆减少了病原体的识别和细胞分裂,而LAB被中性粒细胞有效清除.
科学领域:
- 兽医免疫学 兽医免疫学
- 牛乳腺炎的发病因子
- 微生物与宿主之间的相互作用
背景情况:
- 非黄金色葡萄球菌 (NASM) 和乳酸细菌 (LAB) 越来越多地与牛乳炎有关.
- 这些细菌与牛免疫细胞相互作用的具体机制尚不完全理解.
研究的目的:
- 研究牛中性粒细胞对NASM (Staphylococcus hominis,Staphylococcus chromogenes) 和LAB (Weissella paramesenteroides) 的不同反应.
- 阐明托尔类受体 (TLR) 和下游信号通路在中性粒细胞对这些病原体的防御中的作用.
主要方法:
- 牛中性粒细胞受到S. hominis,S. chromogenes和W. paramesenteroides的挑战.
- 分析了细胞亡,TLR表达 (TLR2,TLR6),细胞亡,细胞内活性氧物种 (ROS) 生产和基因表达 (TLRs,细胞亡相关基因).
主要成果:
- 中性粒细胞在NASM和LAB挑战时显示出最小的亡.
- 挑战后TLR2表达高,但NASM的TLR6表达低.
- 与LAB相比,对NASM,特别是S.染色体,中性细胞化和杀死受损.
- 细胞内ROS的产生始终很高.
- S. 染色体诱导TLRs,ROS和两种亲/反亡基因的上调,与W. paramesenteroides在特定的亡相关基因表达上有所不同 (CASP9,BCL2).
结论:
- 葡萄球菌染色体积极操纵牛中性粒细胞的功能,包括细胞化和基因表达,以逃避免疫清除.
- 与NASM相比,乳酸细菌 (Weissella paramesenteroides) 对中性粒细胞亡的影响不那么大.
- 不同的TLR6识别和细胞能力有助于中性粒细胞对NASM和LAB的反应的有效性不同.
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