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人体皮肤作为维护Mycobacterium leprae和麻风研究的活体模型

Natália Aparecida de Paula^1,2,3, Marcel Nani Leite², Daniele Ferreira de Faria Bertoluci⁴

¹Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto14049-900, Brazil.

Tropical medicine and infectious disease

|June 26, 2024

概括

研究人员开发了一种新型的人体皮肤扩展模型 (hOSEC) 来研究Mycobacterium leprae (M. leprae),这是导致麻风的细菌. 这种ex vivo模型成功地维持了M. leprae60天,为麻风研究提供了一个有前途的替代方案.

科学领域:

传染性疾病传染性疾病
微生物学微生物学
皮肤病学皮肤病学
实验病理学实验病理学

背景情况:

在体外培养Mycobacterium leprae (M. leprae) 仍然是不可能的.
现有的动物模型对研究麻风的临床方面和细菌与人类宿主相互作用有局限性.
开发替代实验模型对于推进麻风研究至关重要.

研究的目的:

为维护和研究M. leprae.标准化一个ex vivo人体皮肤扩展模型 (hOSEC).
评估hOSEC作为麻风研究中的动物模型的潜在替代品.
在hOSEC系统中评估M. leprae的生存能力,传染性和宿主-病原体相互作用.

主要方法:

使用人体皮肤扩展剂的hOSEC模型的标准化.
将M. leprae注射到实验剂中,并在DMEM介质中维持长达60天.
使用RT-PCR-16SrRNA和细胞因子基因表达分析 (TGF-β,IL-10,IL-8,TNF-α) 评估扩展物.
在小鼠中使用Shepard模型评估恢复的M. leprae活力和传染性.

主要成果:

所有hOSEC的实验对象通过RT-PCR-16SrRNA检测M. leprae呈阳性.
从实验物中恢复的M. leprae在小鼠中表现出活力和传染性,在28日的阳性率为80%.
观察到细胞因子基因表达的不同模式,以回应活与死M. leprae.

结论:

hOSEC模型成功地维持了M. leprae的活力60天,使其能够与宿主系统相互作用.
这种ex vivo模型代表了研究M. leprae生物学,宿主相互作用和药物敏感性的重大进步.
在麻风研究中,hOSEC模型为动物模型提供了一个有希望的替代方案.

关键词:

这是一种Mycobacterium leprae细菌.用于动物的替代品.在ex vivo皮肤培养过程中这里是hOSECEC.这是一种麻风病,就是麻风病.组织培养技术组织培养技术

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