miR-122-5p通过向COL4A1促进NR8383中的2.5-诱导的亡
Yize Sun1, Ke Sun1, Zhenhua Ma1
1Department of Veterinary Medicine, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
Toxics
|June 26, 2024
概括
畜牧场 微粒物质2.5 (PM2.5) 暴露会诱导大鼠巨细胞的细胞损伤和亡. 在这个过程中,米R-122-5p通过向COL4A1发挥关键作用,提供潜在的治疗点.
科学领域:
- 环境健康 环境健康
- 分子生物学分子生物学
- 细胞毒理学细胞毒理学
背景情况:
- 颗粒物2.5 (PM2.5) 对健康构成重大风险,但畜牧场PM2.5的影响和miRNA在细胞损伤中的作用仍然不清楚.
- 了解农业来源的PM2.5对动物和人类健康的影响至关重要.
研究的目的:
- 为了研究牛舍PM2.5对大鼠膜巨细胞NR8383.3的影响.
- 阐明miR-122-5p在NR8383细胞中PM2.5诱导的亡中的作用.
- 确定miR-122-5p的目标基因及其参与细胞损伤途径.
主要方法:
- 在实验室模型中,使用NR8383细胞暴露在牛棚PM2.5.5的环境中.
- 通过流细胞计和Hoechst 33342/PI染色来量化亡.
- 生物信息分析以确定miR-122-5p的目标,然后进行分子实验.
主要成果:
- 暴露在PM2.5下降了细胞活力,并增加了细胞亡,这取决于剂量和时间.
- miR-122-5p模拟了上调的亲亡蛋白 (Bcl-xL) 和分裂的caspase-3,同时抑制了抗亡蛋白 (Bcl-2).
- COL4A1被确定为miR-122-5p的标;它的过度表达通过PI3K/AKT/NF-κB通路抑制了PM2.5诱导的亡.
结论:
- miR-122-5p通过向COL4A1.1,在NR8383细胞中调解PM2.5诱导的亡.
- COL4A1对PM2.5诱导的细胞损伤起着保护作用.
- 这些发现突出了miR-122-5p和COL4A1作为减轻PM2.5相关健康问题的潜在治疗点.
相关概念视频
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...


