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高覆盖度的UHPLC-MS/MS分析67种菌毒素在血中的男性不孕症暴露研究

Xiao Ning^1,2, Lulu Wang², Jia-Sheng Wang³

¹School of Food Science and Technology, International Joint Laboratory on Food Safety, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi 214122, China.

|June 26, 2024

概括

人类血分析显示,对40种真菌毒素的广泛联合暴露,不孕男性的发病率更高,特别是甲素A和氨酸.

科学领域:

环境化学环境化学
毒理学毒理学毒理学
分析化学分析化学

背景情况:

菌毒素是重要的食物传播疾病原体,对人类健康构成威胁.
在体内与真菌毒素的同时暴露仍未得到充分研究.
对真菌毒素的生物监测对于公共卫生评估至关重要.

研究的目的:

开发一种敏感和全面的方法来量化人体血中的多种真菌毒素.
为了研究人类血中真菌毒素的同时暴露模式.
探索毒素共同暴露与男性不孕症之间的潜在关联.

主要方法:

开发了一种新的分析策略,用于量化人体血中的67种真菌毒素.
采用同位素稀释质谱法 (IDMS) 和内部标准量化.
菌毒素被按物理化学特性分类,以优化提取和检测.

主要成果:

该方法表现出高灵敏度 (LOD 0.0010.5 μg/L) 和良好的精度 (1.811.9% RSD).
在184个血样本中检测到40种真菌毒素,包括24种新兴的真菌毒素.
不育的男性对多种真菌毒素的共同暴露显著增加,包括 ochratoxin A, ochratoxin B 和 citrinin.

结论:

开发的方法适用于菌毒素的生物监测.
人类广泛暴露于传统和新兴真菌毒素得到证实.
在不育的男性中增加的真菌毒素共同暴露需要进一步调查它们在男性不育方面的作用.

关键词:

在UHPLC-MS/MS中使用.新出现的真菌毒素.人类生物监测生物监测男人的不孕不育症.菌类毒素 (mycotoxin) 是一种有毒的物质.

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