在接触粘合剂粘合剂后对人类牙纸干细胞的细胞毒性影响
Antonella Castellanos Caroprese1, Alexa Nicolle Chinchilla Navarrete1, Sandra Janeth Gutiérrez Prieto1
1Centro de Investigaciones Odontológicas, Facultad de Odontología, Pontificia Universidad Javeriana, BogotáD.C., Colombia.
Brazilian dental journal
|June 26, 2024
概括
牙粘合剂粘合剂在被1毫米牙屏障隔离时对人类牙纸干细胞 (hDPSC) 产生最小的细胞毒性作用. 拉曼光谱检测到DNA损伤,表明其评估细胞毒性的潜力.
科学领域:
- 生物材料科学 生物材料科学
- 牙科材料 牙科材料
- 细胞生物学 细胞生物学
背景情况:
- 牙粘合剂粘合剂在纸组织上的细胞毒性仍然是不确定的.
- 评估这些材料在临床应用中的安全性对于患者的治疗结果至关重要.
研究的目的:
- 在体内模拟牙纤维干细胞对粘合剂的暴露.
- 为了评估通过牙屏障的自蚀刻粘合剂和两步粘合剂的安全性.
主要方法:
- 人类牙纤维干细胞 (hDPSCs) 通过牙屏障暴露在两种类型的粘合剂中.
- 用MTT测定和光显微镜评估了细胞毒性.
- 拉曼光谱法用于分析细胞结构变化和DNA损伤.
主要成果:
- 在所有测试的技术中没有观察到显著的细胞毒性作用.
- 细胞活力仍然很高 (约90%),牙屏障至少为1毫米.
- 拉曼光谱检测了hDPSC暴露在双阶段结合剂中的结构性DNA损伤.
结论:
- 当存在足够的牙屏障时,牙粘合剂在临床使用中是安全的.
- 拉曼光谱作为一个有价值的补充工具,用于检测微妙的细胞毒性超出简单的生存能力测试.
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