相关实验视频

Updated: Jun 23, 2025

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Published on: March 26, 2018

缓慢复制的白血病细胞代表了白血病干细胞群体,具有高细胞表面CD74表达

Huan Li¹, Zhijie Cao¹, Yiming Liu¹

¹State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Tianjin Key Laboratory of Cell Therapy for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China.

Molecular oncology

|June 26, 2024

概括

持久性白血病干细胞 (LSCs) 驱动化疗耐药性. 这项研究使用PKH染料识别了循环缓慢的LSC,并发现CD74是准这些耐药细胞的潜在表面标记物.

科学领域:

血液学血液学血液学
癌症生物学癌症生物学
干细胞研究干细胞研究

背景情况:

静态白血病干细胞 (LSCs) 在治疗后持续存在,导致化疗耐药性和白血病预后不佳.
识别这些静止的LSC对于制定有效的根除策略至关重要.

研究的目的:

在白血病模型中识别和表征静止性白血病干细胞 (LSCs).
调查CD74作为LSCs潜在的表面标记物的作用.

主要方法:

利用PKH26细胞追踪染料在体内识别标签保留,循环缓慢的白血病细胞.
分析了细胞周期阶段 (G0),殖民地形成,白血病发病潜力和PKH保留细胞的化疗耐药性.
检查了白血病细胞中的基因表达和细胞表面标记物概况,重点是CD74.

主要成果:

鉴定了一种循环缓慢,保留标签的白血病细胞群体,具有增强的殖民地形成和白血病发病潜力.
保持PKH的细胞表现出更高的化疗耐药性和偏好的局部化到骨内骨髓.
在这些LSC和CD34+人类白血病细胞中,HLAII类基因相容性抗原马链 (CD74) 的高度表达.
与CD74细胞相比,CD74+白血病细胞表现出较高的干细胞特性.

结论:

已识别的循环缓慢,PKH保留细胞代表了白血病干细胞 (LSC) 群体.
CD74是LSC的有希望的表面标记物,为白血病治疗提供了潜在的标.

关键词:

CD74 CD74 CD74 CD74 CD74 CD74 CD74 CD74 CD74 CD74 CD74 CD74 急性骨髓性白血病 (AML) 是一种急性骨髓性白血病.抗化学疗法耐药性抗化学疗法耐药性白血病的干细胞是什么?静止是一种静止.自行更新的自我更新.

更多相关视频

Author Spotlight: Identification and Isolation of Quiescent Leukemia Stem Cells from Zebrafish T-ALL

Author Spotlight: Identification and Isolation of Quiescent Leukemia Stem Cells from Zebrafish T-ALL

Published on: July 19, 2024

Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia

Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia

Published on: February 21, 2021

相关概念视频

Cancer Stem Cells and Tumor Maintenance

Cancer Stem Cells and Tumor Maintenance

Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...

Multipotency of Hematopoietic Stem Cells

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...