相关实验视频

Updated: Jun 23, 2025

Lateral Root Inducible System in Arabidopsis and Maize

Lateral Root Inducible System in Arabidopsis and Maize

Published on: January 14, 2016

MYB2和MYB108通过与Arabidopsis thaliana中的LBD29相互作用来调节横向根部发育

Feng Zhang¹, Junxia Wang¹, Tingting Ding^1,2

¹The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao, 266237, China.

Journal of integrative plant biology

|June 26, 2024

概括

辅酶信号调节侧向根部发育. 这项研究揭示了MYB2和MYB108转录因子与LBD29相互作用,形成控制素诱导的Arabidopsis thaliana横向根生长的复合体.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
发展生物学发展生物学

背景情况:

由Auxin响应因子7 (ARF7) 介导的auxin信号传递对于横向根 (LR) 发育至关重要.
侧面器官边界域 (LBD) 蛋白质,如LBD29,是ARF7的下游标,并参与LR形成,可能通过二分化.

研究的目的:

调查LBD29是否形成蛋白质复合体来调节辅酶诱导的横向根部发育.
确定LBD29的新型交互伙伴,并阐明它们在LR发展中的作用.

主要方法:

酵母两杂交测试以确定蛋白质相互作用.
对Arabidopsis thaliana突变物 (例如,SRDX融合的MYB2) 和过度表达线的分析.
辅助治疗和横向根形成的量化.
对下游目标的基因表达分析,如皮破坏因子1 (CDEF1).

主要成果:

确定了MYB转录因子MYB2和MYB108作为LBD29.9的新型相互作用伙伴.
无论是MYB2还是MYB108,都以ARF7依赖的方式被auxin诱导.
干扰MYB2功能损害了辅酶诱导的LR形成和LBD29促进LR发育的能力.
过度表达MYB2或MYB108增强了LR形成,但这种效果取决于LBD29.29的存在.
发现MYB2-LBD29和MYB108-LBD29复合体促进了参与LR发育的脂酶CDEF1的表达.

结论:

MYB2和MYB108与LBD29相互依赖,以调节辅酶介导的横向根部发育.
这些MYB-LBD29监管模块在ARF7的下游作用,为LR形成的复杂控制提供了新的见解.

关键词:

在ARF7中,ARF7在ARF7中使用.LBD2929 这是一个很好的方法.这就是MYB1088.这就是MYB2的原因.辅助剂辅助剂辅助剂侧面的根是侧面的根.

更多相关视频

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Published on: May 14, 2020

相关概念视频

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

Short-distance Transport of Resources

Short-distance Transport of Resources

Short-distance transport refers to transport that occurs over a distance of just 2-3 cells, crossing the plasma membrane in the process. Small uncharged molecules, such as oxygen, carbon dioxide, and water, can diffuse across the plasma membrane on their own. In contrast, ions and larger molecules require the assistance of transport proteins due to their charge or size. Transport across membranes also occurs within individual cells, playing a variety of essential roles for the plant as a whole.

Primary and Secondary Growth in Roots and Shoots

Primary and Secondary Growth in Roots and Shoots

Vascular plants, which account for over 90% of the Earth’s vegetation, all undergo primary growth—which lengthens roots and shoots. Many land plants, notably woody plants, also undergo secondary growth—which thickens roots and shoots.