可注射的活凝与嵌入益生菌作为一种新的策略,用于打击多方面的病原体伤口感染
Siyuan Tao1, Sixuan Zhang1, Kongchang Wei1,2
1Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, St. Gallen, 9014, Switzerland.
Advanced healthcare materials
|June 26, 2024
概括
ProGel是一种新型的活体水凝,利用益生菌细菌对抗伤口感染. 这种可注射材料有效地减少有害病原体,防止生物膜的形成,为传统抗生素提供了一个有希望的替代品.
科学领域:
- 生物材料科学 生物材料科学
- 微生物学 微生物学
- 伤口治愈研究研究 伤口治愈研究
背景情况:
- 伤口感染是一个重大的临床挑战,通常由抗生素耐药性加剧.
- 目前的治疗方法难以克服耐药性病原体和生物膜形成.
研究的目的:
- 开发和评估ProGel,一种可注射的活水凝,包含益生菌Lactobacillus plantarum,用于治疗伤口感染.
- 评估ProGel的抗菌疗效,机械性能和体内性能.
主要方法:
- 通过使用双注射器系统将乳酸菌植物菌 (Lactobacillus plantarum) 陷入凝基质中来制造ProGel.
- 评估了机械性能,储存期间的细菌活力和对关键病原体的抗菌活性.
- 使用人类皮肤纤维细胞评估了细胞相容性,并在受感染的人类皮肤伤口模型上测试了疗效.
主要成果:
- ProGel表现出强大的机械和自我愈合特性,保持高益生菌活力 (>80%起初,一周后>70%).
- 观察到Pseudomonas aeruginosa (7-log),Staphylococcus aureus (3-7-log) 和Candida albicans (40-70%) 的显着减少,这些病例的发生率都比较低.
- 在受感染的人类皮肤伤口模型中,ProGel成功抑制了生物膜的形成,并显示出良好的细胞兼容性.
结论:
- ProGel是一个创新的可注射活凝系统,用于治疗复杂的伤口感染.
- 该系统有效地提供具有广谱抗菌活性和生物膜预防能力的益生菌.
- 这种方法为挑战性伤口感染提供了传统抗生素治疗的有希望的替代方案.
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