在成人和新生儿小鼠的腰部内注射
Marina Stavrou1, Elena Georgiou1, Kleopas A Kleopa1,2
1Neuroscience Department, The Cyprus Institute of Neurology and Genetics, Nicosia, Cyprus.
Current protocols
|June 26, 2024
概括
这项研究详细介绍了小鼠的腰部内注射,这是一种最小的侵入性方法,用于将埃文斯蓝色染料和AAV9-EGFP等物质输送到中枢和外周神经系统.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 手术技巧 手术技巧
背景情况:
- 内注射是一种临床相关的方法,用于将治疗药物输送到中枢神经系统 (CNS).
- 在小鼠中进行内注射在技术上要求很高,需要专门的专业知识.
- 这种技术允许直接进入脑脊液 (CSF) 并随后在中枢神经系统和周围神经系统 (PNS) 中进行分布.
研究的目的:
- 为成年和新生小鼠提供腰部内注射的详细,逐步的方案.
- 为了证明注射物质 (埃文斯蓝色染料和AAV9-EGFP) 在中枢神经系统,脑后神经系统和全身循环中的生物分布.
- 建立一种可复制的方法,通过腰部内注射输送各种溶液.
主要方法:
- 在成年 (2个月大) 和新生儿 (产后第10天) C57BL / 6小鼠的L5-L6脊椎间空间的腰椎内注射.
- 使用埃文斯蓝色染料和携带增强绿色光蛋白 (AAV9-EGFP) 的腺相关病毒血清型9.
- 组织采集和准备用于埃文斯蓝色染料扩散分析,免疫组织化学和载体基因组拷贝数分析.
主要成果:
- 成功的腰部内注射导致注射材料的广泛生物分布.
- 该技术允许直接进入中枢神经系统的副体和外围神经.
- 当正确执行时,该方法是最少的侵入性和无干扰性.
结论:
- 腰部内注射是一种多功能和有效的技术,用于将溶液输送到小鼠的中枢神经系统和脑后神经系统.
- 该协议有助于研究神经系统疾病和基因疗法.
- 描述的方法在临床上可转化,适用于各种研究应用.
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