表面等离子体共振 (SPR) 应用于研究各种蛋白质 - 连接体相互作用
Dana M Burris1,2, Samuel W Gillespie1,2, Emma Joy Campbell1
1Bond Life Sciences Center, University of Missouri, Columbia, Missouri.
Current protocols
|June 26, 2024
概括
表面等离子共振 (SPR) 是一种测量生物分子结合 afinities 的无标签技术. 这项研究详细介绍了基于SPR的三个协议,用于分析蛋白质 - 连接体相互作用,包括病原体检测和药物发现.
科学领域:
- 生物化学 生化学
- 生物物理学的生物物理.
- 分析化学 分析化学
背景情况:
- 准确地确定蛋白质 - 配体结合亲缘关系对于蛋白质组学和药物发现至关重要.
- 现有的技术需要精确,高通量测试来选潜在的候选人.
- 表面等离子共振 (SPR) 为生物分子相互作用的直接测量提供了一种无标签的方法.
研究的目的:
- 描述使用基于SPR的测试进行精确分析蛋白质-连接体相互作用的三个协议.
- 突出SPR在各种应用中的实用性,包括病原体检测和药物发现.
- 引入本地化SPR (LSPR) 作为现场研究的便携式替代方案.
主要方法:
- 在金属表面上固定一个连接体,并将分析剂溶液流过它.
- 通过反射光来测量折射率变化,以实时检测绑定事件.
- 利用SPR成像和LSPR来提高便携性和现场检测能力.
主要成果:
- 证明SPR用于比较病毒逆转录酶多形态的结合亲和力.
- 使用SPR.成功选原始植物提取物的蛋白质结合剂使用SPR.
- 开发基于LSPR的抗原检测与抗体结合金纳米粒子.
结论:
- 基于SPR的测定提供了精确的实时数据,生物分子动力学和特异性.
- SPR及其变体 (SPR成像,LSPR) 是多功能工具,适用于从基础研究到诊断的各个领域.
- 对于活植物研究和便携式检测系统来说,LSPR具有优势.
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