Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

4.0K
The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
 
Most enzymes...
4.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Research progress of cuproptosis, ferroptosis, apoptosis, and autophagy in knee osteoarthritis.

Frontiers in cell and developmental biology·2026
Same author

Development and validation of a simplified dynamic nomogram for preoperative prediction of microvascular invasion in hepatocellular carcinoma.

European journal of gastroenterology & hepatology·2026
Same author

Sex-linked helicases DDX3X and DDX3Y regulate G-quadruplex-associated stress in neurons.

Cell death & disease·2026
Same author

Bisphenol A exacerbates diabetic foot ulcers through disruption of immune microenvironment and repair processes: a multi-omics analysis of environmental exposure mechanisms.

Drug and chemical toxicology·2026
Same author

The effect of multi-disciplinary treatment on surgical outcomes and kinematic gait patterns in patients undergoing primary unilateral knee unicompartmental arthroplasty: a retrospective cohort study.

Frontiers in nutrition·2026
Same author

Case Report: Report of 2 cases of periprosthetic joint infection caused by Brucella after joint replacement and literature review.

Frontiers in surgery·2026

相关实验视频

Updated: Jun 23, 2025

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
10:01

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

Published on: December 4, 2017

12.2K

一种经济高效的基于血红素的人工酶可以用于实际应用.

Dehui Qiu1, Fangni He1, Yuan Liu1

  • 1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|June 26, 2024
PubMed
概括

研究人员开发了一种新型的人工酶,即组装激活的黑酶 (AA-heminzyme),模仿自然酶. 这种具有成本效益的纳米材料为废水处理和生物标志物检测等应用提供了卓越的稳定性和效率.

关键词:
这是一个G-四重复式.人工酶是一种人造酶.这里是hemin hemin.希斯蒂丁类型的类似物

更多相关视频

GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization
11:38

GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

Published on: October 24, 2011

15.5K
Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening
05:44

Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening

Published on: January 26, 2024

826

相关实验视频

Last Updated: Jun 23, 2025

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
10:01

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

Published on: December 4, 2017

12.2K
GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization
11:38

GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

Published on: October 24, 2011

15.5K
Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening
05:44

Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening

Published on: January 26, 2024

826

科学领域:

  • 生物模拟化学是生物模拟化学.
  • 纳米材料科学 纳米材料科学
  • 催化剂是一种催化剂.

背景情况:

  • 自然酶提供有价值的催化功能,但在稳定性和成本方面存在局限性.
  • 纳米材料提供了一个创建具有增强性能的人工酶的平台.

研究的目的:

  • 设计和表征一种新型的人工酶,组装激活的黑酶 (AA-heminzyme),使用黑,胺类同类物和G-四重复DNA.
  • 与天然酶相比,评估AA-heminzyme的催化效率,稳定性和经济可行性.

主要方法:

  • 包括分子对接和量子化学计算在内的in silico调查.
  • 在体外催化试验中,比较了AA-heminzyme与胡卜过氧化酶 (HRP).
  • 评估热稳定性,可回收性和成本效益.

主要成果:

  • AA-heminzyme的催化效率与HRP相似,其转化率 (kcat) 为115s-1.1.
  • 人工酶表现出卓越的热稳定性,可回收性和可编辑性.
  • AA-heminzyme的催化成本低于HRP的10%的成本.

结论:

  • AA-heminzyme是一种高效,稳定和经济可行的仿生催化剂.
  • 它的战略潜力在工业废水整治和成本效益高的生物标志物检测方面得到证实,例如谷氨分析.