在严格的标准的应用中对结构问题进行评估,以评估精子形态
Leonie van den Hoven1, Netty J van Vrouwerff2, Ineke M Dijkstra2
1Fertility Laboratory Department of Obstetrics and Gynaecology, Radboud University Medical Centre, Nijmegen, The Netherlands.
Andrology
|June 26, 2024
概括
精子形态评估显示了解释的高度变化,影响了精液分析的一致性. 这突出了当前世卫组织手册对关键形态标准的定义的问题.
科学领域:
- 生殖医学 生殖医学
- 安德罗学与人类学
- 实验室质量控制和控制
背景情况:
- 精子形态评估是精液分析的标准,历史上与受孕有关.
- 最近的焦点转移到形态学作为精子生成和男性健康的标志物.
- 由于解释挑战和范式转变,许多实验室已经停止了形态评估.
研究的目的:
- 在荷兰外部质量控制 (EQC) 计划中确定高结果变异性的精子形态标准.
- 根据特定的形态学标准,分析随时间变化的趋势.
主要方法:
- 评估了2015-2020年的EQC结果,涉及40-60个实验室,评估了72张精子细胞照片.
- 使用了WHO5 (2010) 中定义的14个形态标准.
- 每个标准的评估变化和时间趋势.
主要成果:
- 诸如体真空和尾部指标等标准显示,实验室间的差异性很低.
- 头部卵度,轮规律性和轴线对齐度表现出最高的变化.
- 随着时间的推移,总体变化趋势略有下降,除了最可变的标准.
结论:
- 在解释精子形态学标准方面存在显著的变异性,导致精液分析结果不一致.
- 世卫组织手册定义和示例中的不完美可能有助于这些解释挑战.
- 标准化标准的解释对于可靠的精子形态评估至关重要.
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