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缺陷工程金属有机框架作为药物治疗的纳米载体:洞察单个粒子水平的细胞内动力学
Ge Huang1,2,3,4, Marcus Winther Dreisler1,2,3,4, Jacob Kæstel-Hansen1,2,3,4
1Department of Chemistry & Nano-Science Center, Copenhagen University, Thorvaldsensvej 40, Frederiksberg, Copenhagen, 1871, Denmark.
Advanced materials (Deerfield Beach, Fla.)
|June 26, 2024
概括
这项研究介绍了光纳米金属有机框架 (nanoMOFs),用于跟踪细胞通路. 了解纳米MOF细胞内动力学对于安全有效的药物输送应用至关重要.
科学领域:
- 材料科学 材料科学 材料科学
- 纳米技术 纳米技术
- 细胞生物学 细胞生物学
背景情况:
- 纳米金属有机框架 (nanoMOFs) 用于药物输送,生物传感和生物成像.
- 纳米MOFs的细胞内途径和命运尚不清楚,这限制了它们的安全应用.
研究的目的:
- 开发一种方法来追踪单个纳米MOFs在活细胞中的时空定位.
- 为了阐明细胞内化的途径和纳米MOFs的细胞内命运.
- 量化评估纳米MOF与内分/溶酶体区间的同位化.
主要方法:
- 合成一个光纳米MOF (UiO-66) 集成ATTO 655.5.
- 密度函数理论计算,以了解染料-节点结合.
- 数以千计的纳米MOF的并行单粒子跟踪.
- 分析纳米MOF本地化和内部化途径的机器学习.
- 开发一种对内分泌/淋巴体区的局部化代理方法.
主要成果:
- 在活细胞中成功跟踪单个纳米MOF,使用光标记UIO-66.6.
- 确定了纳米MOF细胞内化通路,并确定它们是否保持细胞外.
- 量化测量纳米MOF与早期/晚期内分泌体和溶解体的同位化.
- 展示了一种新的局部化策略,以减轻与信号相关的偏差.
结论:
- 通过单颗粒追踪了解纳米MOF细胞内动力学对于优化药物输送至关重要.
- 开发的光纳米MOF和跟踪方法提供了关于细胞内纳米MOF行为的见解.
- 准确评估纳米MOF细胞内命运对于确保安全有效的纳米医学应用至关重要.
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