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使用基于质谱的N-glycoproteomics分析突变起源的糖化增益

Hailun Yang¹, Zhixin Tian¹

¹School of Chemical Science & Engineering, Shanghai Key Laboratory of Chemical Assessment and Sustainability, Tongji University, Shanghai, China.

Rapid communications in mass spectrometry : RCM

|June 26, 2024

概括

这项研究开发了一种质谱管道,用于分析乳腺癌干细胞中与突变相关的糖化 (GoG) 增益. 工作流确定了N-glycopeptides,并描述了N-glycosylation异质性,为癌症进展提供了洞察力.

科学领域:

生物化学和分子生物学
蛋白质组学是指蛋白质组学.
癌症研究癌症研究

背景情况:

已经建立了一个一般的N-glycoproteomics分析管道,用于在完整的N-glycopeptide分子水平上表征与突变相关的糖化化 (GoG).
这条管道产生了N-糖化的全面的位置和结构信息.

研究的目的:

专注于基于质谱的N-glycoproteomics分析工作流程的突变起源的糖化增益 (GoG).
为了从父母 (MCF-7 CSCs) 和抗胺素 (MCF-7/ADR CSCs) 乳腺癌干细胞中识别GoG完整的N-糖.
描述与突变相关的特定位点和结构的N-糖化模式.

主要方法:

利用基于质谱的N-糖蛋白组学工作流.
建立了GoG完整的N-葡萄糖数据库,包括2701种蛋白质和6709种人类N-葡萄糖.
使用GPSeeker搜索引擎采用特定于站点和结构的N-糖蛋白组学工作流.

主要成果:

分别从MCF-7 CSC和MCF-7/ADR CSC中鉴定了87和94 GoG完整的N-糖 (FDR ≤1%).
在MCF-7 CSC和MCF-7/ADR CSC中鉴定了37个和35个与GoG相关的完整N-糖蛋白.
在GoG完整的N-糖蛋白中观察到N-糖化酶的微和宏异质性.

结论:

集成的网站和结构特定的N-glycoproteomics方法为GoG表征提供了通用的工作流.
这种方法揭示了GoG.的N-糖和N-糖结构信息.
对突变起源的GoG的分析可以扩展到其他N-糖蛋白质组系统,包括临床样本.

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