相关实验视频

Updated: Jun 23, 2025

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

克里斯普尔/Pepper-tDeg:一个实时成像系统能够使用一个单指导RNA进行非重复的基因组位置分析

Meng Chen¹, Xing Huang¹, Yakun Shi¹

¹Guangdong Provincial Key Laboratory of Sensing Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-Sen University, Sun Yat-Sen University, Shenzhen, 518107, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|June 26, 2024

概括

研究人员开发了CRISPR/Pepper-tDeg,这是一个先进的基因组成像系统. 该系统显著提高了信号噪声比,可用于可视化活细胞中重复和非重复的DNA序列.

科学领域:

分子生物学分子生物学
遗传学遗传学是一个
细胞生物学细胞生物学

背景情况:

基于CRISPR的基因组成像能够对活细胞中的DNA进行时空可视化.
现有系统面临的挑战是信号噪声比 (SNR) 低,特别是对于非重复的基因组位点.
改进的成像技术对于理解基因组组织和动态至关重要.

研究的目的:

开发一个高效的基于CRISPR的基因组成像系统,增强SNR.
为了能够对重复和非重复的基因组位置进行敏感的成像.
减少对基因组组织和动态研究的技术障碍.

主要方法:

工程 CRISPR sgRNA 脚手架与降氧结合的 Pepper 体.
使用的原蛋白与Tat衍生的降解子域 (tDeg) 融合.
实施了对 sgRNA 和原蛋白的依赖目标的稳定性开关.

主要成果:

与CRISPR/MS2-MCP相比,CRISPR/Pepper-tDeg在端粒成像方面取得了5倍高的SNR.
成功证明了同时标记和跟踪端粒和中端粒.
扩展了用于非重复序列成像的系统,并使用分裂光蛋白改进了SNR.

结论:

克里斯帕/Pepper-tDeg提供了高SNR的敏感和高效的基因组成像.
该系统简化了sgRNA设计和等离子体构造,降低了技术障碍.
显示了生物研究,临床诊断和治疗的巨大潜力.

关键词:

克里斯普尔-卡斯9是什么意思化蛋白质是一种化蛋白质.基因组位置标记标签活细胞成像活细胞成像不重复的序列是不重复的序列.

更多相关视频

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Published on: November 1, 2024

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...