相关实验视频

Updated: Jun 23, 2025

Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration

Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration

Published on: September 27, 2022

在高生育率与低生育率的牛精子中的piRNA表达模式

Nicholas Werry¹, Stewart J Russell², Raamkumaar Sivakumar¹

¹Department of Biomedical Sciences, The University of Guelph, Guelph, Ontario, Canada.

Systems biology in reproductive medicine

|June 26, 2024

概括

雄牛精子中的PIWI相互作用RNAs (piRNAs) 在很大程度上保持在各种生育水平上,这表明它们在维持基因组稳定性和精子活力方面发挥着至关重要的作用. 这些piRNAs积极抑制可转移元素,影响早期胚胎发育和受精能力.

科学领域:

生殖生物学生殖生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
基因组学就是基因组学.

背景情况:

与PIWI相互作用的RNAs (piRNAs) 对于生殖线发育和对可移植元素的基因组防御至关重要.
精子piRNAs在将表观遗传信息传递给胚胎方面发挥着作用.
荷尔斯坦公牛的异形不孕症提供了一个研究精子质量的piRNA功能的模型.

研究的目的:

来自不同生育能力的公牛的精子中的piRNA表达和基因组集群的特征.
研究piRNA配置文件,可移植元素抑制和生育结果之间的关系.
评估精子piRNAs对早期胚胎发生的影响.

主要方法:

RNA测序以分析公牛精子中的piRNA种群.
主要组成部分和差异表达分析以比较生育率组.
生物信息映射用于识别piRNA来源和可转移元素目标.

主要成果:

皮RNA种群显示高和低生育率公牛之间的高度相似性,表明强烈的保护.
证据表明"乒乓球"活动证实了活跃的可转移元素向在两组.
精子piRNAs,主要是29-30个核酸,起源于226个基因组集群,并针对多个可转移元素家族.
在早期胚胎发生过程中,向基因表达减少,这表明它在表观遗传调节中的作用.

结论:

精子piRNA配置文件高度保存,对于可行的精子生产和基因组完整性至关重要.
精子中可转移元素的PiRNA介导的抑制会影响早期胚胎基因表达.
精子生成中的piRNAs的表观遗传调节可能会影响精子质量和受精能力.

关键词:

雄牛的生育能力雄牛的生育能力表观遗传学是指表观遗传学.男人的生育能力男人的生育能力这就是piRNAs.很小的RNAs是小的RNAs.精子RNARNARNA精子RNARNA精子RNA精子RNA精子RNA精子RNA精子RNA精子RNA精子RNA精子

更多相关视频

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Published on: January 30, 2017

Analysis of Epididymal Protein Synthesis and Secretion

Analysis of Epididymal Protein Synthesis and Secretion

Published on: August 25, 2018

相关概念视频

Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...

Fertilization

Fertilization

During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...