酸性部分化 (pH<6) 促进了超高效的短流转化:实验验证和基因组洞察
Fangzhai Zhang1, Ziyi Du1, Jiahui Wang1
1National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
Water research
|June 26, 2024
概括
这项研究开发了一种稳定的酸性部分化 (PN) 过程,实现了高氨氧化率和对干扰的弹性. 它确定了关键的微生物转变和功能基因,推动了这种高效的转化.
科学领域:
- 环境微生物学环境微生物学
- 生物技术是生物技术.
- 污水处理 污水处理 污水处理
背景情况:
- 部分化 (PN) 节能,但面临着不稳定的酸盐积累和冲击等挑战.
- 现有的PN过程在运行稳定性和反应速度缓慢方面扎.
研究的目的:
- 建立一个稳定的酸性部分化 (PN) 过程,具有自我维持的低pH值.
- 研究涉及酸性PN的微生物群落和功能基因.
- 确定有助于过程稳定性和效率的因素.
主要方法:
- 在pH值为5.36的酸性PN过程的培养,持续120天.
- 监测亚酸盐积累率 (NAR),氨氧化率 (AOR) 和操作参数.
- 使用16S rRNA基因测序对微生物社区结构的分析.
- 功能基因 (例如,amoB) 的量化和其表达的评估.
主要成果:
- 取得了稳定的NAR>97.9%和0.81kg/m3·d的超高AOR.
- 在各种运营冲击下,NAR>77.8%的弹性被证明.
- 观察到耐酸性细菌的主导地位,如Candidatus Nitrosoglobus和Acidobacteriota等.
- 确定了amoB作为关键的功能基因,并揭示了影响酸盐氧化细菌 (NOB) 的机制.
结论:
- 酸性PN提供了稳定高效的转化途径.
- 特定的微生物转移和功能性基因表达是过程稳定的关键.
- 了解NOB抑制机制可以进一步优化PN过程.
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