相关实验视频
Updated: May 12, 2026

08:23
Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes
Published on: December 28, 2011
17.4K
单细胞转录组测序揭示了糖尿病诱导的心肌细胞的代谢变化和微环境变化
Weiyu Zhou1, Haiqiao Yu1, Shuang Yan1
1Department of Endocrine and Metabolic Diseases, The Fourth Affiliated Hospital of Harbin Medical University, No.37, Yiyuan Street, Nangang District, Harbin, Heilongjiang 150000, China.
Computational biology and chemistry
|June 26, 2024
概括
糖尿病 (DM) 导致心脏细胞 (CMs) 发生显著变化,改变它们的沟通和脂质代谢. 这些细胞转移有助于糖尿病心脏中的心肌纤维化.
科学领域:
- 心血管生物学 心血管生物学
- 代谢障碍 代谢障碍 代谢障碍
- 单细胞基因组学 单细胞基因组学
背景情况:
- 糖尿病是一种慢性代谢障碍,由高血糖定义.
- 心脏细胞 (CMs) 对于心脏功能至关重要,并可能受到DM的影响.
- 了解DM中的细胞异质性和通信对于治疗开发至关重要.
研究的目的:
- 分析糖尿病小鼠心中的CMs中的细胞异质性和通信变化.
- 为了确定心脏中糖尿病影响的分子通路和相互作用.
- 为了研究CM变化的作用在糖尿病诱导的心肌纤维化.
主要方法:
- 利用来自对照和糖尿病小鼠心脏的单细胞RNA测序数据 (GSE213337).
- 采用基因组丰富分析 (GSEA) 和基因组变异分析 (GSVA) 来对CMs中的差异表达基因 (DEG) 进行功能丰富.
- 进行了细胞通信分析,以确定改变的信号通路和连接体-受体相互作用.
主要成果:
- 确定了17种不同的细胞类型,CM比率增加,表明糖尿病的扩散.
- 在CM中发现了1144个DEG,GSEA/GSVA表明脂肪代谢增强.
- 揭示了改变的CM通信:纤维细胞/LECs的受体增加,ECCs/pericytes的受体减少.
- 观察到GAS和ANGPTL水平下降,与途径分析一致.
- 在CM中观察到联体受体相互作用的变化,例如vegfc-vegfr2和angptl1.
结论:
- CMs在DM中表现出显著的异质性,有助于高血糖引起的心肌纤维化.
- 改变CMs中的细胞通信和新陈代谢是糖尿病心肌病的关键特征.
- 这些发现突出了糖尿病心脏病管理的潜在目标.
相关概念视频
Cell Specific Gene Expression
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Carbohydrate Metabolism
Carbohydrates are polymers composed of molecules containing atoms of carbon, hydrogen and oxygen. One gram of carbohydrate can provide four kilo-calories of energy, which makes it the most efficient instant energy source.
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in the...
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in the...
Type II Diabetes I: Introduction
Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterized by insulin resistance, in which target tissues such as the liver, muscle, and adipose tissue respond poorly to insulin. It is also associated with inadequate compensatory insulin secretion, where pancreatic β-cells fail to produce sufficient insulin. Together, these abnormalities lead to persistent hyperglycemia.EtiologyT2DM develops through a complex interaction of genetic predisposition and environmental or...

